<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Turner, O C</style></author><author><style face="normal" font="default" size="100%">Roberts, A D</style></author><author><style face="normal" font="default" size="100%">Frank, A A</style></author><author><style face="normal" font="default" size="100%">Phalen, S W</style></author><author><style face="normal" font="default" size="100%">McMurray, D M</style></author><author><style face="normal" font="default" size="100%">Content, J</style></author><author><style face="normal" font="default" size="100%">Denis, O</style></author><author><style face="normal" font="default" size="100%">D'Souza, S</style></author><author><style face="normal" font="default" size="100%">Tanghe, A</style></author><author><style face="normal" font="default" size="100%">Huygen, K</style></author><author><style face="normal" font="default" size="100%">Orme, I M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Lack of protection in mice and necrotizing bronchointerstitial pneumonia with bronchiolitis in guinea pigs immunized with vaccines directed against the hsp60 molecule of Mycobacterium tuberculosis.</style></title><secondary-title><style face="normal" font="default" size="100%">Infect Immun</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Infect Immun</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">BCG Vaccine</style></keyword><keyword><style  face="normal" font="default" size="100%">Bronchiolitis</style></keyword><keyword><style  face="normal" font="default" size="100%">Chaperonin 60</style></keyword><keyword><style  face="normal" font="default" size="100%">Guinea Pigs</style></keyword><keyword><style  face="normal" font="default" size="100%">Lung</style></keyword><keyword><style  face="normal" font="default" size="100%">mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycobacterium tuberculosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Necrosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Pneumonia, Bacterial</style></keyword><keyword><style  face="normal" font="default" size="100%">Tuberculosis, Pulmonary</style></keyword><keyword><style  face="normal" font="default" size="100%">Vaccination</style></keyword><keyword><style  face="normal" font="default" size="100%">Vaccines, DNA</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000 Jun</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">3679</style></number><volume><style face="normal" font="default" size="100%">68</style></volume><pages><style face="normal" font="default" size="100%">3674-9</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In this study, the hsp60 and hsp70 heat shock protein antigens of Mycobacterium tuberculosis were tested as potential vaccine candidates, using purified recombinant protein antigens or antigens encoded in the form of a DNA plasmid vaccine. Guinea pigs vaccinated with a mixture of the two proteins showed no evidence of resistance to low-dose aerosol challenge infection and quickly developed severe lung damage characterized by necrotizing bronchointerstitial pneumonia and bronchiolitis. As a result, we turned instead to a DNA vaccination approach using a plasmid encoding the hsp60 antigen of M. tuberculosis. Although immunogenic in mice, vaccination with plasmid DNA encoding hsp60 was not protective in that model or in the guinea pig model and again gave rise to similar severe lung damage. This study seriously questions the safety of vaccines against tuberculosis that target highly conserved heat shock proteins.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/10816527?dopt=Abstract</style></custom1><section><style face="normal" font="default" size="100%">3674</style></section></record></records></xml>