<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">David Fretin</style></author><author><style face="normal" font="default" size="100%">Whatmore, Adrian M</style></author><author><style face="normal" font="default" size="100%">Sascha Al Dahouk</style></author><author><style face="normal" font="default" size="100%">Neubauer, Heinrich</style></author><author><style face="normal" font="default" size="100%">Garin-Bastuji, Bruno</style></author><author><style face="normal" font="default" size="100%">Albert, David</style></author><author><style face="normal" font="default" size="100%">Van Hessche, Mieke</style></author><author><style face="normal" font="default" size="100%">Ménart, Marie</style></author><author><style face="normal" font="default" size="100%">Godfroid, Jacques</style></author><author><style face="normal" font="default" size="100%">Walravens, Karl</style></author><author><style face="normal" font="default" size="100%">P Wattiau</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Brucella suis identification and biovar typing by real-time PCR.</style></title><secondary-title><style face="normal" font="default" size="100%">Vet Microbiol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Vet. Microbiol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Brucella suis</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism, Genetic</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008 Oct 15</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">131</style></volume><pages><style face="normal" font="default" size="100%">376-85</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Fast and accurate identification of Brucella suis at the biovar level is an important issue for public health laboratories because some of the biovars that infect suidae (boars and pigs) are pathogenic for humans while others are not. Since classical biovar typing methods are often time-consuming, hard to standardize and require high-level biosafety containment, methodological improvements are desirable. This article describes new single nucleotide polymorphism (SNP) signatures for the rapid identification and biovar characterization of B. suis. These SNPs were included together with previously described ones in real-time PCR assays applicable to low-biosafety conditions. Allelic profiles unique for each B. suis biovar were defined and the most relevant signatures were determined on a collection of 137 field strains of worldwide origin characterized previously. Biovars assigned with both present and classical methods were globally consistent except for some biovar 3 field strains which matched the allelic profile of biovar 1.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3-4</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/18499359?dopt=Abstract</style></custom1></record></records></xml>