<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ferreira, Helena Lage</style></author><author><style face="normal" font="default" size="100%">Fabienne Rauw</style></author><author><style face="normal" font="default" size="100%">Pirlot, Jean François</style></author><author><style face="normal" font="default" size="100%">Reynard, Frédéric</style></author><author><style face="normal" font="default" size="100%">Thierry van den Berg</style></author><author><style face="normal" font="default" size="100%">Bublot, Michel</style></author><author><style face="normal" font="default" size="100%">Bénédicte Lambrecht</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparison of single 1-day-old chick vaccination using a Newcastle disease virus vector with a prime/boost vaccination scheme against a highly pathogenic avian influenza H5N1 challenge.</style></title><secondary-title><style face="normal" font="default" size="100%">Avian Pathol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Avian Pathol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals, Newborn</style></keyword><keyword><style  face="normal" font="default" size="100%">Chickens</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Vectors</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunity, Humoral</style></keyword><keyword><style  face="normal" font="default" size="100%">Influenza A Virus, H5N1 Subtype</style></keyword><keyword><style  face="normal" font="default" size="100%">Influenza in Birds</style></keyword><keyword><style  face="normal" font="default" size="100%">Newcastle disease virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Poultry Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Vaccines</style></keyword><keyword><style  face="normal" font="default" size="100%">Virus Shedding</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">43</style></volume><pages><style face="normal" font="default" size="100%">68-77</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Avian influenza (AI) vaccines should be used as part of a whole comprehensive AI control programme. Vectored vaccines based on Newcastle disease virus (NDV) are very promising, but are so far licensed in only a few countries. In the present study, the immunogenicity and protection against a highly pathogenic H5N1 influenza challenge were evaluated after vaccination with an enterotropic NDV vector expressing an H5 haemagglutinin (rNDV-H5) in 1-day-old specific pathogen free chickens inoculated once, twice or once followed by a heterologous boost with an inactivated H5N9 vaccine (iH5N9). The heterologous prime/boost rNDV-H5/iH5N9 combination afforded the best level of protection against the H5N1 challenge performed at 6 weeks of age. Two rNDV-H5 administrations conferred a good level of protection after challenge, although only a cellular H5-specific response could be detected. Interestingly, a single administration of rNDV-H5 gave the same level of protection as the double administration but without any detectable H5-specific immune response. In contrast to AI immunity, a high humoral, mucosal and cellular NDV-specific immunity could be detected up to 6 weeks post vaccination after using the three different vaccination schedules. NDV-specific mucosal and cellular immune responses were slightly higher after double rNDV-H5 vaccination when compared with single inoculation. Finally, the heterologous prime/boost rNDV-H5/iH5N9 combination induced a broader detectable immunity including systemic, mucosal and cellular AI and NDV-specific responses.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/24320551?dopt=Abstract</style></custom1></record></records></xml>