<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Letellier, Carine</style></author><author><style face="normal" font="default" size="100%">Boxus, Mathieu</style></author><author><style face="normal" font="default" size="100%">Rosar, Laurent</style></author><author><style face="normal" font="default" size="100%">Toussaint, Jean-François</style></author><author><style face="normal" font="default" size="100%">Walravens, Karl</style></author><author><style face="normal" font="default" size="100%">S. Roels</style></author><author><style face="normal" font="default" size="100%">Meyer, Gilles</style></author><author><style face="normal" font="default" size="100%">Letesson, Jean-Jacques</style></author><author><style face="normal" font="default" size="100%">Pierre Kerkhofs</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.</style></title><secondary-title><style face="normal" font="default" size="100%">Vaccine</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Vaccine</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Bronchoalveolar Lavage Fluid</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">CD8-Positive T-Lymphocytes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Proliferation</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunization, Secondary</style></keyword><keyword><style  face="normal" font="default" size="100%">Interferon-gamma</style></keyword><keyword><style  face="normal" font="default" size="100%">Lung</style></keyword><keyword><style  face="normal" font="default" size="100%">Nucleocapsid Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmids</style></keyword><keyword><style  face="normal" font="default" size="100%">Pneumonia</style></keyword><keyword><style  face="normal" font="default" size="100%">Respiratory Syncytial Virus Infections</style></keyword><keyword><style  face="normal" font="default" size="100%">Respiratory Syncytial Virus Vaccines</style></keyword><keyword><style  face="normal" font="default" size="100%">Respiratory Syncytial Virus, Bovine</style></keyword><keyword><style  face="normal" font="default" size="100%">Vaccines, DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Vaccines, Subunit</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008 Sep 02</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">26</style></volume><pages><style face="normal" font="default" size="100%">4840-8</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Respiratory syncytial virus (RSV) is a major cause of respiratory disease in both cattle and young children. Despite the development of vaccines against bovine (B)RSV, incomplete protection and exacerbation of subsequent RSV disease have occurred. In order to circumvent these problems, calves were vaccinated with the nucleocapsid protein, known to be a major target of CD8(+) T cells in cattle. This was performed according to a DNA prime-protein boost strategy. The results showed that DNA vaccination primed a specific T-cell-mediated response, as indicated by both a lymphoproliferative response and IFN-gamma production. These responses were enhanced after protein boost. After challenge, mock-vaccinated calves displayed gross pneumonic lesions and viral replication in the lungs. In contrast, calves vaccinated by successive administrations of plasmid DNA and protein exhibited protection against the development of pneumonic lesions and the viral replication in the BAL fluids and the lungs. The protection correlated to the cell-mediated immunity and not to the antibody response.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">37</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/18644416?dopt=Abstract</style></custom1></record></records></xml>