<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vaerewijck, M J M</style></author><author><style face="normal" font="default" size="100%">Sabbe, K</style></author><author><style face="normal" font="default" size="100%">Julie Baré</style></author><author><style face="normal" font="default" size="100%">Spengler, H-P</style></author><author><style face="normal" font="default" size="100%">Favoreel, H W</style></author><author><style face="normal" font="default" size="100%">Houf, K</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Assessment of the efficacy of benzalkonium chloride and sodium hypochlorite against Acanthamoeba polyphaga and Tetrahymena spp.</style></title><secondary-title><style face="normal" font="default" size="100%">J Food Prot</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J. Food Prot.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acanthamoeba</style></keyword><keyword><style  face="normal" font="default" size="100%">Benzalkonium Compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Survival</style></keyword><keyword><style  face="normal" font="default" size="100%">Disinfectants</style></keyword><keyword><style  face="normal" font="default" size="100%">Dose-Response Relationship, Drug</style></keyword><keyword><style  face="normal" font="default" size="100%">Flow Cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Food Parasitology</style></keyword><keyword><style  face="normal" font="default" size="100%">Food Preservation</style></keyword><keyword><style  face="normal" font="default" size="100%">Sodium Hypochlorite</style></keyword><keyword><style  face="normal" font="default" size="100%">Tetrahymena</style></keyword><keyword><style  face="normal" font="default" size="100%">Time Factors</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012 Mar</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">75</style></volume><pages><style face="normal" font="default" size="100%">541-6</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The efficacy of benzalkonium chloride and sodium hypochlorite against Acanthamoeba polyphaga and two Tetrahymena spp. was determined based on the European Standard EN 1276:2009 suspension test. Trophozoite viability was assessed by determination of the membrane integrity using flow cytometry as a fast screening technique. Bovine serum albumin was added to simulate clean (0.3 g/liter) and dirty (3 g/liter) conditions. Benzalkonium chloride caused cell lysis at concentrations above 50 mg/liter under clean and dirty conditions. A concentration of 50 mg of free chlorine per liter had a strong biocidal effect on acanthamoebae and tetrahymenae after 15 min under clean and dirty conditions. Our results suggest that benzalkonium chloride and sodium hypochlorite were effective against the three microorganisms at concentrations commonly applied in the food industry.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22410229?dopt=Abstract</style></custom1></record></records></xml>