<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vangoitsenhoven, Roman</style></author><author><style face="normal" font="default" size="100%">Rondas, Dieter</style></author><author><style face="normal" font="default" size="100%">Inne Crèvecoeur</style></author><author><style face="normal" font="default" size="100%">D'Hertog, Wannes</style></author><author><style face="normal" font="default" size="100%">Baatsen, Pieter</style></author><author><style face="normal" font="default" size="100%">Masini, Matilde</style></author><author><style face="normal" font="default" size="100%">Mirjana Andjelkovic</style></author><author><style face="normal" font="default" size="100%">Joris Van Loco</style></author><author><style face="normal" font="default" size="100%">Matthys, Christophe</style></author><author><style face="normal" font="default" size="100%">Mathieu, Chantal</style></author><author><style face="normal" font="default" size="100%">Overbergh, Lut</style></author><author><style face="normal" font="default" size="100%">Van der Schueren, Bart</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Foodborne cereulide causes beta-cell dysfunction and apoptosis.</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS One</style></secondary-title><alt-title><style face="normal" font="default" size="100%">PLoS ONE</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoptosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cercopithecus aethiops</style></keyword><keyword><style  face="normal" font="default" size="100%">COS Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Depsipeptides</style></keyword><keyword><style  face="normal" font="default" size="100%">Food Microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Glucose</style></keyword><keyword><style  face="normal" font="default" size="100%">Hep G2 Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Insulin</style></keyword><keyword><style  face="normal" font="default" size="100%">Insulin-Secreting Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Islets of Langerhans</style></keyword><keyword><style  face="normal" font="default" size="100%">mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice, Inbred C57BL</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondria</style></keyword><keyword><style  face="normal" font="default" size="100%">rats</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><pages><style face="normal" font="default" size="100%">e104866</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;b&gt;AIMS/HYPOTHESIS: &lt;/b&gt;To study the effects of cereulide, a food toxin often found at low concentrations in take-away meals, on beta-cell survival and function.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS: &lt;/b&gt;Cell death was quantified by Hoechst/Propidium Iodide in mouse (MIN6) and rat (INS-1E) beta-cell lines, whole mouse islets and control cell lines (HepG2 and COS-1). Beta-cell function was studied by glucose-stimulated insulin secretion (GSIS). Mechanisms of toxicity were evaluated in MIN6 cells by mRNA profiling, electron microscopy and mitochondrial function tests.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS: &lt;/b&gt;24 h exposure to 5 ng/ml cereulide rendered almost all MIN6, INS-1E and pancreatic islets apoptotic, whereas cell death did not increase in the control cell lines. In MIN6 cells and murine islets, GSIS capacity was lost following 24 h exposure to 0.5 ng/ml cereulide (P&lt;0.05). Cereulide exposure induced markers of mitochondrial stress including Puma (p53 up-regulated modulator of apoptosis, P&lt;0.05) and general pro-apoptotic signals as Chop (CCAAT/-enhancer-binding protein homologous protein). Mitochondria appeared swollen upon transmission electron microscopy, basal respiration rate was reduced by 52% (P&lt;0.05) and reactive oxygen species increased by more than twofold (P&lt;0.05) following 24 h exposure to 0.25 and 0.50 ng/ml cereulide, respectively.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS/INTERPRETATION: &lt;/b&gt;Cereulide causes apoptotic beta-cell death at low concentrations and impairs beta-cell function at even lower concentrations, with mitochondrial dysfunction underlying these defects. Thus, exposure to cereulide even at concentrations too low to cause systemic effects appears deleterious to the beta-cell.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/25119564?dopt=Abstract</style></custom1></record></records></xml>