<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Peeters, L E J</style></author><author><style face="normal" font="default" size="100%">De Mulder, T</style></author><author><style face="normal" font="default" size="100%">Van Coillie, E</style></author><author><style face="normal" font="default" size="100%">Huygens, J</style></author><author><style face="normal" font="default" size="100%">Smet, A</style></author><author><style face="normal" font="default" size="100%">Daeseleire, E</style></author><author><style face="normal" font="default" size="100%">Dewulf, J</style></author><author><style face="normal" font="default" size="100%">Hein Imberechts</style></author><author><style face="normal" font="default" size="100%">Butaye, P</style></author><author><style face="normal" font="default" size="100%">Haesebrouck, F</style></author><author><style face="normal" font="default" size="100%">Croubels, S</style></author><author><style face="normal" font="default" size="100%">Heyndrickx, M</style></author><author><style face="normal" font="default" size="100%">Rasschaert, G</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by crosscontaminated feed.</style></title><secondary-title><style face="normal" font="default" size="100%">J Appl Microbiol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J. Appl. Microbiol.</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2017 Aug 11</style></date></pub-dates></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;b&gt;AIMS: &lt;/b&gt;The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l(-1) ), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an ex vivo model of the porcine caecum.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS AND RESULTS: &lt;/b&gt;A DOX-resistant, tet(A)-carrying, porcine commensal E. coli strain (EC 682) was cultivated for 6 days in the porcine caecum model under different conditions (0, 1 and 4 mg l(-1) DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n = 454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4 mg l(-1) DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4 mg l(-1) DOX compared to medium without DOX.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS: &lt;/b&gt;Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E. coli, and promote the selection of transconjugants in a porcine caecum model.&lt;/p&gt;&lt;p&gt;&lt;b&gt;SIGNIFICANCE AND IMPACT OF THE STUDY: &lt;/b&gt;Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.&lt;/p&gt;</style></abstract><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/28799283?dopt=Abstract</style></custom1></record></records></xml>