<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Zarev, Yancho</style></author><author><style face="normal" font="default" size="100%">Tania Naessens</style></author><author><style face="normal" font="default" size="100%">Mart Theunis</style></author><author><style face="normal" font="default" size="100%">Elgorashi, Esameldin</style></author><author><style face="normal" font="default" size="100%">Apers, Sandra</style></author><author><style face="normal" font="default" size="100%">Iliana Ionkova</style></author><author><style face="normal" font="default" size="100%">Luc Verschaeve</style></author><author><style face="normal" font="default" size="100%">Luc Pieters</style></author><author><style face="normal" font="default" size="100%">Nina Hermans</style></author><author><style face="normal" font="default" size="100%">Kenn Foubert</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro antigenotoxic activity, in silico ADME prediction and protective effects against aflatoxin B1 induced hepatotoxicity in rats of an Erythrina latissima stem bark extract</style></title><secondary-title><style face="normal" font="default" size="100%">Food and Chemical Toxicology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antigenotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Erythrina latissima</style></keyword><keyword><style  face="normal" font="default" size="100%">In vivo antihepatotoxic activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Leguminosae</style></keyword><keyword><style  face="normal" font="default" size="100%">Prenylated flavonoids</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">31-08-2019</style></date></pub-dates></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Stem bark of Erythrina latissima E. Mey (Leguminosae) contains a wide range of prenylated flavonoids able to counteract the genotoxic properties of aflatoxin B1 (AFB1). Thus, the hypothesis was raised that E. latissima stem bark extracts (ELBE) may counteract the in vivo hepatotoxic effects of aflatoxins, contaminants in food and feed. An HPLC-DAD method was developed and validated to determine the level of flavonoid aglycones (11.82%) and glycosides (16.17%). ADME, pharmacokinetic and drug-likeness assessment of major flavonoids of ELBE, using the web tool SwissADME, showed good oral bioavailability. The protective effect of ELBE against AFB1 induced genotoxicity in the Vitotox assay after metabolic activation was confirmed (IC50 of 44.32 μg/ml), followed by evaluation of its inhibitory effect on hepatotoxicity in rats induced by the same agent. Male Wistar rats were orally treated with ELBE (20 mg/kg, 50 mg/kg and 100 mg/kg) or curcumin (500 mg/kg) combined with piperine (20 mg/kg) - positive control, for 8 days prior to AFB1 exposure (1 mg/kg). The ELBE group showed a decreased activity of ALP and γ-GT compared to the AFB1 group. Histopathological examination of the liver demonstrated ameliorative effects of ELBE. Thus, ELBE could have a protective effect against hepatotoxins such as AFB.&lt;/p&gt;
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