Medical analysis request

Medical analysis request [1]

Sciensano is recognized by INAMI-RIZIV [2] for the analyses of clinical microbiology which are performed in our national reference centres. It are mainly second-line analyses (confirmations, typings, etc.) although also first-line analyses for less usual pathogens are performed. Sciensano is accredited ISO15189 [3].

How to request a human microbiological analysis?

Pathogen(s)

Pathogen(s)	Description	Material(s)	Accreditation	Turnaround time (in days)	Contact person(s)	Request and administrative forms
Bacillus anthracis [5]	Bacillus anthracis (human) [6]	Serum	No	7	Marcella Mori [7],	formulaire de demande analyse humain Leptospira - Charbon - Morve - Tularemie-Parapoxvirus [8] analyseaanvraagformulier humaan Leptospira - Miltvuur - Kwade Droes - Tularemie -Parapoxvirus [9]
Bacillus cereus [10]	Detection of enterotoxins of Bacillus cereus (strain) [11]	Strains	No	6	foodmicro@sciensano.be [12]	Aanvraagformulier Staphylococcus aureus en Bacillus cereus [13] Formulaire de demande Staphylococcus aureus et Bacillus cereus [14] Fiche du test enterotoxines Staphyloccocus aureus et Baccilus cereus [15] Testfiche enterotoxines Staphylococcus aureus en Bacillus cereus [16] Request typing of foodborne strains [17]
Bacillus cereus [18]	Detection of Bacillus cereus [19]	faeces	No	6	foodmicro@sciensano.be [12]	Aanvraagformulier Staphylococcus aureus en Bacillus cereus [13] Formulaire de demande Staphylococcus aureus et Bacillus cereus [14] Fiche du test enterotoxines Staphyloccocus aureus et Baccilus cereus [15] Testfiche enterotoxines Staphylococcus aureus en Bacillus cereus [16]
Bacillus cereus [20]	Molecular identification of genetic determinants for cereulide (emetic toxin of Bacillus cereus) [21]	Strains	No	6	foodmicro@sciensano.be [12]	Aanvraagformulier Staphylococcus aureus en Bacillus cereus [13] Formulaire de demande Staphylococcus aureus et Bacillus cereus [14] Fiche du test enterotoxines Staphyloccocus aureus et Baccilus cereus [15] Testfiche enterotoxines Staphylococcus aureus en Bacillus cereus [16]
Bacillus cereus [22]	Molecular identification of genes encoding Bacillus cereus enterotoxins (diarrhea) [23]	faeces, Food, strain	No	9
Bacterial strains [24]	Antimicrobial susceptibility (Broth microdilution) [25]	Bacterial strain	No	30	Pieter-Jan Ceyssens [26], yersinia@sciensano.be [27]	Aanvraagformulier [28] , Formulaire de demande Yersinia [29]
Bacterial strains [30]	Genotyping via whole genome sequencing [31]	Bacterial strain	No	25	Wesley Mattheus [32], yersinia@sciensano.be [27]	Aanvraagformulier Yersinia [29] , Formulaire de demande Yersinia [29]
Bordetella pertussis [33]	IgG quantification (ELISA) against Pertussis Toxin (whooping cough) (sera) [34]	Serum	[3]	7	Caroline Rodeghiero [35], , Isabelle Desombere [36], immunomail@sciensano.be [37]	Aanvraagformulier Bordetella pertussis serologie [38] Aanvraagformulier Bordetella pertussis Serologie [39] Formulaire de demande Pertussis Serologie [40] , Formulaire de demande Bordetella pertussis sérologie [41] Aanvraagformulier Bordetella pertussis Serologie [39] Formulaire de demande Pertussis Serologie [40]
Brucella spp. [42]	Buffered plate agglutination test [43]	Liquid, Lyophilized serum, Plasma, Serum	No	4	Marcella Mori [7],
Brucella spp. [44]	Buffered plate agglutination test + EDTA [45]	Lyophilized serum, Serum	No	4	Marcella Mori [7],
Brucella spp. [46]	Identification Brucella spp. [47]	Bacterial strain, Cerebrospinal fluid, EDTA Blood, Hemoculture, Liquid	[3]	3	Marcella Mori [7],
Brucella spp. [48]	Isolation Brucella spp. [49]	Bacterial strain, Biological material, Cerebrospinal fluid, EDTA Blood, Hemoculture	[3]	12	Marcella Mori [7],
Brucella spp. [50]	PCR Brucella spp. [51]	Biological material, DNA, Bacterial strain	[3]	4	Marcella Mori [7],
Brucella spp. [52]	Rose bengale test [53]	Biological material, Cerebrospinal fluid, Paired sera, Plasma, Serum	[3]	4	Marcella Mori [7],
Brucella spp. [54]	ELISA (Sciensano) Brucella spp. [55]	Biological material, Lyophilized serum, Plasma, Serum	[3]	4	Marcella Mori [7],
Burkholderia mallei [56]	Complement fixation test glanders and melioidosis [57]	Serum	No	5	David Fretin [58], , Marcella Mori [7],	formulaire de demande analyse humain Leptospira - Charbon - Morve - Tularemie-Parapoxvirus [8] analyseaanvraagformulier humaan Leptospira - Miltvuur - Kwade Droes - Tularemie -Parapoxvirus [9]
Clostridium botulinum [59]	Detection of botulinum toxins (reference method in vivo) [60]	faeces	[3]	10	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Aanvraagformulier Clostridium botulinum [63] , Formulaire de demande Clostridium botulinum [64]
Clostridium botulinum [65]	Detection of botulinum toxins (reference method in vivo) [66]	Serum	[3]	10	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Aanvraagformulier Clostridium botulinum [63] , Formulaire de demande Clostridium botulinum [64]
Clostridium botulinum [67]	Detection of neurotoxigenic Clostridium botulinum and related - types A, B, E and F (realtime PCR) [68]	faeces	[3]	15	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Aanvraagformulier Clostridium botulinum [69] , Formulaire de demande Clostridium botulinum [70]
Clostridium botulinum [71]	Detection of C. botulinum (in vivo reference method) [72]	faeces	[3]	15	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Aanvraagformulier Clostridium botulinum [63] , Formulaire de demande Clostridium botulinum [64]
Clostridium perfringens [73]	Toxinotype of C. perfringens (realtime PCR) [74]	strain	No	15	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Analyseaanvraag enterotoxinogene Clostridium perfringens [75] Demande d'analyse Clostridium perfringens entérrotoxinogène [76]
Clostridium perfringens [77]	Detection of C. perfringens (realtime PCR) [78]	faeces	[3]	15	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Analyseaanvraag enterotoxinogene Clostridium perfringens [75] Demande d'analyse Clostridium perfringens entérrotoxinogène [76]
Clostridium perfringens [79]	Enumeration of C. perfringens in faeces [80]	faeces	[3]	10	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Analyseaanvraag enterotoxinogene Clostridium perfringens [75] Demande d'analyse Clostridium perfringens entérrotoxinogène [76]
Clostridium perfringens [81]	Detection Enterotoxine from C. perfringens in feces [82]	faeces	No	7	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Aanvraagformulier C. perfringens [83]
Clostridium perfringens [84]	EUCAST disk diffusion test for C. perfringens [85]	Strains	No	10	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Aanvraagformulier C. perfringens [83]
Clostridium tetani [86]	In vitro determination of antitetanic antibodies [87]	Serum	[3]	10	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Formulaire de demande tetanos [88] Aanvraagformulier tetanus [89] fiche du test tetanos [90] testfiche tetanos [91]
Clostridium tetani [92]	Detection of tetanus toxin (in vivo method) [93]	Serum	No	10	Tom Van Nieuwenhuysen [61], botulisme@sciensano.be [62]	Formulaire de demande tetanos [88] Aanvraagformulier tetanus [89] fiche du test tetanos [90] testfiche tetanos [91]
Coagulase Positive Staphylococci [94]	Detection of coagulase positive staphylococci [95]	faeces	No	6	foodmicro@sciensano.be [12]	Aanvraagformulier Staphylococcus aureus en Bacillus cereus [13] Formulaire de demande Staphylococcus aureus et Bacillus cereus [14] Fiche du test enterotoxines Staphyloccocus aureus et Baccilus cereus [15] Testfiche enterotoxines Staphylococcus aureus en Bacillus cereus [16]
Corynebacterium diphtheriae [96]	Quantification of anti-Corynebacterium diphtheriae toxin IgG antibodies (ELISA) [97]	Serum	[3]	14	Isabelle Desombere [36], ImmunoMail@sciensano.be [98]	Aanvraagformulier Corynebacterium [99] , Formulaire de demande Corynebacterium [100]
Coxiella spp. [101]	PCR Coxiella burnetii (Q-fever) [102]	EDTA Blood, Heart, Liquid, Pool van swabs, Puncture fluid, Serum, Skin, Throat swab	[3]	5	Marcella Mori [7],
Dermatophytes [103]	Detection and identification of dermatophytes (Tinea) [104]	Swabs	No	15	medical_mycology@sciensano.be [105]	Formulaire de demande Tinea [106] Aanvraagformulier Tinea [107]
Filamentous fungi [108], Yeast [108], Molds [108]	MALDI-TOF MS identification of yeasts and molds (clinical fungi) [109]	strain	No	10	medical_mycology@sciensano.be [105]	Formulaire de demande identification mycologie [110] Aanvraagformulier identificatie mycologie [111]
Filamentous fungi [112], Yeast [112], Molds [112]	Identification by DNA sequencing (clinical fungi) [113]	strain	No	20	medical_mycology@sciensano.be [105]	Formulaire de demande identification mycologie [110] Aanvraagformulier identificatie mycologie [111]
Francisella tularensis [114]	Isolation and identification Francisella tularensis [115]	Bacterial strain, Biological material, Ganglia, Intestine, Kidney, Liver, Lung, Pool organs, Spleen, Swab	No	10	David Fretin [58], , Marcella Mori [7],	formulaire de demande analyse humain Leptospira - Charbon - Morve - Tularemie-Parapoxvirus [8] analyseaanvraagformulier humaan Leptospira - Miltvuur - Kwade Droes - Tularemie -Parapoxvirus [9]
Francisella tularensis [116]	ELISA Francisella tularensis [117]	Cerebrospinal fluid, Serum	No	5	Marcella Mori [7],	formulaire de demande analyse humain Leptospira - Charbon - Morve - Tularemie-Parapoxvirus [8] analyseaanvraagformulier humaan Leptospira - Miltvuur - Kwade Droes - Tularemie -Parapoxvirus [9]
Francisella tularensis [118]	Rapid Test Francisella [119]	Serum	No	3	Marcella Mori [7],	formulaire de demande analyse humain Leptospira - Charbon - Morve - Tularemie-Parapoxvirus [8] analyseaanvraagformulier humaan Leptospira - Miltvuur - Kwade Droes - Tularemie -Parapoxvirus [9]
Gastrovirus [120]	Detection of gastrovirus [121]	faeces	No	Not applicable	foodmicro@sciensano.be [12]	Formulaire de demande Gastrovirus -Sapovirus [122] Aanvraagformulier Gastrovirus -Sapovirus [123] Fiche du test Gastrovirus - Sapovirus [124] Testfiche Gastrovirus - Sapovirus [125]
Hepatitis A virus [126]	HAV virus detection by qPCR [127]	Serum	[3]	7	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis A virus [132]	Genotyping of HAV virus [133]	Serum/Salive	No	30	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis A virus [134]	Ig tot detection of HAV virus by Elisa [135]	Serum	[3]	7	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis A virus [136]	IgM detection of HAV virus by Elisa [137]	Serum	[3]	7	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis B virus [138]	HBV PCR (CUSL) [139]	EDTA Blood, Serum	No	14	benoit.kabamba@uclouvain.be [140]	Aanvraagformulier Hepatitis [141] Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131] , Formulaire de demande Hepatitis [142] Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis C virus [143]	Total Ig detection of HCV virus by Immunoblot [144]	Serum	[3]	10	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis C virus [145]	HCV resistance testing: NS3A sequencing (CUSL) [146]	EDTA Blood, Serum	No	20	benoit.kabamba@uclouvain.be [140]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis C virus [147]	HCV resistance testing : NS5A sequencing (CUSL) [148]	EDTA Blood, Serum	No	20	benoit.kabamba@uclouvain.be [140]	Aanvraagformulier Hepatitis [141] Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis C virus [149]	HCV resistance testing : NS5B sequencing (CUSL) [150]	EDTA Blood, Serum	No	20	benoit.kabamba@uclouvain.be [140]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis D virus [151]	Detection of hepatitis D virus by qPCR [152]	Serum, Tested Here	[3]	14	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier Hepatitis [153] Formulaire de demande hépatites [131]
Hepatitis D virus [154]	Ig tot detection of HDV virus by Elisa [155]	Serum, Tested Here	[3]	14	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis E virus [156]	IgG detection of HEV virus by Elisa [157]	Serum, Tested Here	[3]	7	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]
Hepatitis E virus [158]	IgM detection of HEV virus by Elisa [159]	Serum, Tested Here	[3]	7	Florence Lemaitre [128], virologie@sciensano.be [129]	Aanvraagformulier hepatitis [130] Formulaire de demande hépatites [131]

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