Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control. [1]
Animal health [2]
Peer reviewed scientific article
SCIENSANO
Authors
Hoffmann, Bernd [3]; Blome, Sandra [4]; Bonilauri, Paolo [5]; Fernández-Piñero, Jovita [6]; Greiser-Wilke, Irene [7]; Andy Haegeman [8]; Isaksson, Mats [9]; F. Koenen [10]; LeBlanc, Neil [11]; Leifer, Immanuel [12]; Le Potier, Marie-Frédérique [13]; Loeffen, Willie [14]; Rasmussen, Thomas Bruun [15]; Stadejek, Tomasz [16]; Ståhl, Karl [17]; Marylène Tignon [18]; Uttenthal, Ase [19]; van der Poel, Wim [20]; Beer, Martin [21]Keywords
Abstract:
The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples. In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical …