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High titre Hog Cholera virus production on Cytodex 3 microcarrier cultures. [1]

Animal health   [2]

Peer reviewed scientific article

SCIENSANO

Authors

Ann Brigitte Cay [3]; De Smet, A [4]; Dubois, N [5]; F. Koenen [6]

Keywords

  1. Animals [7]
  2. Cells, Cultured [8]
  3. Classical swine fever virus [9]
  4. Dextrans [10]
  5. Virus Cultivation [11]

Abstract:

In an attempt to produce Hog Cholera virus (HCV) preparations of high titre, optimal growth and trypsinization conditions of PK-15 microcarrier cell cultures were defined. Infecting a PK-15 Cytodex 3 microcarrier culture with HCV increased the yield of virus more than 10 times compared with conventional monolayer culture in Roux flasks.
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Associated health topics:

Animal health [2]
Source URL:https://sciensano.be/en/biblio/high-titre-hog-cholera-virus-production-cytodex-3-microcarrier-cultures

Links
[1] https://sciensano.be/en/biblio/high-titre-hog-cholera-virus-production-cytodex-3-microcarrier-cultures [2] https://sciensano.be/en/health-topics/animal-health [3] https://sciensano.be/en/people/brigitte-cay/biblio [4] https://sciensano.be/en/biblio?f%5Bauthor%5D=39909&f%5Bsearch%5D=De%20Smet%2C%20A [5] https://sciensano.be/en/biblio?f%5Bauthor%5D=39912&f%5Bsearch%5D=Dubois%2C%20N [6] https://sciensano.be/en/biblio?f%5Bauthor%5D=28872&f%5Bsearch%5D=F.%20Koenen [7] https://sciensano.be/en/biblio?f%5Bkeyword%5D=423&f%5Bsearch%5D=Animals [8] https://sciensano.be/en/biblio?f%5Bkeyword%5D=29256&f%5Bsearch%5D=Cells%2C%20Cultured [9] https://sciensano.be/en/biblio?f%5Bkeyword%5D=29721&f%5Bsearch%5D=Classical%20swine%20fever%20virus [10] https://sciensano.be/en/biblio?f%5Bkeyword%5D=19644&f%5Bsearch%5D=Dextrans [11] https://sciensano.be/en/biblio?f%5Bkeyword%5D=22926&f%5Bsearch%5D=Virus%20Cultivation