<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kris De Clercq</style></author><author><style face="normal" font="default" size="100%">Ilse De Leeuw</style></author><author><style face="normal" font="default" size="100%">Verheyden, B</style></author><author><style face="normal" font="default" size="100%">Vandemeulebroucke, E</style></author><author><style face="normal" font="default" size="100%">Vanbinst, T</style></author><author><style face="normal" font="default" size="100%">Herr, C</style></author><author><style face="normal" font="default" size="100%">Estelle Méroc</style></author><author><style face="normal" font="default" size="100%">Bertels, G</style></author><author><style face="normal" font="default" size="100%">Steurbaut, N</style></author><author><style face="normal" font="default" size="100%">Miry, C</style></author><author><style face="normal" font="default" size="100%">De Bleecker, K</style></author><author><style face="normal" font="default" size="100%">Maquet, G</style></author><author><style face="normal" font="default" size="100%">Bughin, J</style></author><author><style face="normal" font="default" size="100%">Saulmont, M</style></author><author><style face="normal" font="default" size="100%">Lebrun, M</style></author><author><style face="normal" font="default" size="100%">Sustronck, B</style></author><author><style face="normal" font="default" size="100%">De Deken, R</style></author><author><style face="normal" font="default" size="100%">Hooyberghs, J</style></author><author><style face="normal" font="default" size="100%">Houdart, P</style></author><author><style face="normal" font="default" size="100%">Raemaekers, M</style></author><author><style face="normal" font="default" size="100%">Mintiens, K</style></author><author><style face="normal" font="default" size="100%">Pierre Kerkhofs</style></author><author><style face="normal" font="default" size="100%">Goris, N</style></author><author><style face="normal" font="default" size="100%">Frank Vandenbussche</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Transplacental infection and apparently immunotolerance induced by a wild-type bluetongue virus serotype 8 natural infection.</style></title><secondary-title><style face="normal" font="default" size="100%">Transbound Emerg Dis</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Transbound Emerg Dis</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Abortion, Veterinary</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals, Newborn</style></keyword><keyword><style  face="normal" font="default" size="100%">Belgium</style></keyword><keyword><style  face="normal" font="default" size="100%">Bluetongue</style></keyword><keyword><style  face="normal" font="default" size="100%">Bluetongue virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Infectious Disease Transmission, Vertical</style></keyword><keyword><style  face="normal" font="default" size="100%">Pregnancy</style></keyword><keyword><style  face="normal" font="default" size="100%">Pregnancy Complications, Infectious</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Viral</style></keyword><keyword><style  face="normal" font="default" size="100%">Serotyping</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008 Oct</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">55</style></volume><pages><style face="normal" font="default" size="100%">352-9</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Until recently, bluetongue (BT) virus (BTV) serotypes reportedly causing transplacental infections were all ascribed to the use of modified live virus strains. During the 2007 BT epidemic in Belgium, a significant increase in the incidence of abortions was reported. A study including 1348 foetuses, newborns and young animals with or without suspicion of BTV infection, was conducted to investigate the occurrence of natural transplacental infection caused by wild-type BTV-8 and to check the immunocompetence of newborns. BTV RNA was present in 41% and 18.5% of aborted foetuses from dams with or without suspected BTV involvement during pregnancy, respectively. The results of dam/calf pairs sampled before colostrum uptake provide evidence of almost 10% transplacental BTV infection in newborns. Apparently immunotolerant calves were found at a level of 2.4%. The current study concludes that the combined serological and real-time PCR (RT-qPCR) result of pregnant dams gives no indication of the infection status of the offspring except in the case of a double negative result. In a group of 109 calves with clinical suspicion of BT, born during the vector-free period, 11% were found to be RT-qPCR positive. The true prevalence was estimated to be 2.3%, indicating the extent of transplacental infection in a group of 733 calves of one to 4 months of age without BT suspicion. Moreover, virus isolation was successful for two newborn calves, emphasizing the need for restricting trade to BT-free regions of pregnant dams possibly infected during gestation, even if they are BTV RT-qPCR negative.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/18673339?dopt=Abstract</style></custom1></record></records></xml>