<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>6</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">De Smet, Jeroen</style></author><author><style face="normal" font="default" size="100%">Hendrix, Hanne</style></author><author><style face="normal" font="default" size="100%">An Van den Bossche</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Martha R.J. Clokie</style></author><author><style face="normal" font="default" size="100%">Andrew Kropinski</style></author><author><style face="normal" font="default" size="100%">Lavigne, Rob</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Phage–Host Protein–Protein Interactions Using Strep-tag® II Purifications</style></title><secondary-title><style face="normal" font="default" size="100%">Phage–Host Protein–Protein Interactions Using Strep-tag® II Purifications</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">affinity purifications; bacteriophage; host protein; mass spectrometry; pseudomonas aeruginosa; phage; protein interactions;</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><number><style face="normal" font="default" size="100%">136</style></number><publisher><style face="normal" font="default" size="100%">Springer New York</style></publisher><pub-location><style face="normal" font="default" size="100%">New York, NY</style></pub-location><volume><style face="normal" font="default" size="100%">1898</style></volume><pages><style face="normal" font="default" size="100%">117 - 136</style></pages><isbn><style face="normal" font="default" size="100%">978-1-4939-8939-3</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;After injecting their genome into the bacterial host cell, bacteriophages need to convert the host metabolism toward efficient phage production. For this, specific proteins have evolved which interact with key host proteins to inhibit, activate or redirect the function of these proteins. Since 70% of the currently annotated phage genes are hypothetical proteins of unknown function, the identification and characterization of these phage proteins involved in host–phage protein–protein interactions remains challenging. Here, we describe a method to identify phage proteins involved in host–phage protein–protein interactions using a combination of affinity purifications and mass spectrometry analyses. A bacterial strain is engineered in which a bacterial target protein is fused to a Strep-tag® II at the C-terminal end. This strain is infected with a specific bacteriophage, followed by an affinity purification of the tagged protein which allows the copurification of all bacterial and phage specific interacting proteins. After SDS-PAGE analysis and an in-gel trypsin digestion, the purified interacting proteins are identified by mass spectrometry analysis. The identification of phage proteins involved in interactions provides first hints toward the elucidation of the biological function of these proteins.&lt;/p&gt;
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