<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">De Smet, Jeroen</style></author><author><style face="normal" font="default" size="100%">Jeroen Wagemans</style></author><author><style face="normal" font="default" size="100%">Natalia Akulenko</style></author><author><style face="normal" font="default" size="100%">Evgeny Klimuk</style></author><author><style face="normal" font="default" size="100%">Subray Hedge</style></author><author><style face="normal" font="default" size="100%">Voet, Marleen</style></author><author><style face="normal" font="default" size="100%">Hendrix, Hanne</style></author><author><style face="normal" font="default" size="100%">Paeshuyse, Jan</style></author><author><style face="normal" font="default" size="100%">Bart Landuyt</style></author><author><style face="normal" font="default" size="100%">Xu, Hua</style></author><author><style face="normal" font="default" size="100%">John Blanchard</style></author><author><style face="normal" font="default" size="100%">Konstantin Severinov</style></author><author><style face="normal" font="default" size="100%">Lavigne, Rob</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Phage-Encoded -Acetyltransferase Rac Mediates Inactivation of  Transcription by Cleavage of the RNA Polymerase Alpha Subunit.</style></title><secondary-title><style face="normal" font="default" size="100%">Viruses</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">acetylomics</style></keyword><keyword><style  face="normal" font="default" size="100%">bacterial shutdown</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteriophage</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2020 09 02</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses. These phages encode a single-subunit RNA polymerase for transcription of their late (structure- and lysis-associated) genes, whereas the bacterial RNA polymerase is used at the earlier stages of infection. Rac mediates the inactivation of bacterial transcription by introducing a specific cleavage in the α subunit of the bacterial RNA polymerase. This cleavage occurs within the flexible linker sequence and disconnects the C-terminal domain, required for transcription initiation from most highly active cellular promoters. To achieve this, Rac likely taps into a novel post-translational modification (PTM) mechanism within the host . From an evolutionary perspective, this novel phage-encoded regulation mechanism confirms the importance of PTMs in the prokaryotic metabolism and represents a new way by which phages can hijack the bacterial host metabolism.&lt;/p&gt;
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