<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hendrix, Hanne</style></author><author><style face="normal" font="default" size="100%">Maria Zimmermann-Kogadeeva</style></author><author><style face="normal" font="default" size="100%">Zimmermann, Michael</style></author><author><style face="normal" font="default" size="100%">Sauer, Uwe</style></author><author><style face="normal" font="default" size="100%">De Smet, Jeroen</style></author><author><style face="normal" font="default" size="100%">Laurens Muchez</style></author><author><style face="normal" font="default" size="100%">Maries Lissens</style></author><author><style face="normal" font="default" size="100%">Ines Staes</style></author><author><style face="normal" font="default" size="100%">Voet, Marleen</style></author><author><style face="normal" font="default" size="100%">Jeroen Wagemans</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">Noben, Jean-Paul</style></author><author><style face="normal" font="default" size="100%">Aertsen, Abram</style></author><author><style face="normal" font="default" size="100%">Lavigne, Rob</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation.</style></title><secondary-title><style face="normal" font="default" size="100%">Cell Rep</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetyltransferases</style></keyword><keyword><style  face="normal" font="default" size="100%">Anti-Bacterial Agents</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteriophages</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon</style></keyword><keyword><style  face="normal" font="default" size="100%">Metabolic Networks and Pathways</style></keyword><keyword><style  face="normal" font="default" size="100%">Metabolome</style></keyword><keyword><style  face="normal" font="default" size="100%">Metabolomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Models, Biological</style></keyword><keyword><style  face="normal" font="default" size="100%">Pseudomonas aeruginosa</style></keyword><keyword><style  face="normal" font="default" size="100%">Quinolones</style></keyword><keyword><style  face="normal" font="default" size="100%">Quorum Sensing</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary Metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Proteins</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2022 02 15</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">38</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The Pseudomonas quinolone signal (PQS) is a multifunctional quorum sensing molecule of key importance to P.&amp;nbsp;aeruginosa. Here, we report that the lytic Pseudomonas bacterial virus LUZ19 targets this population density-dependent signaling system by expressing quorum sensing targeting protein (Qst) early during infection. We demonstrate that Qst interacts with PqsD, a key host quinolone signal biosynthesis pathway enzyme, resulting in decreased levels of PQS and its precursor 2-heptyl-4(1H)-quinolone. The lack of a functional PqsD enzyme impairs LUZ19 infection but is restored by external supplementation of 2-heptyl-4(1H)-quinolone, suggesting that LUZ19 exploits the PQS system for successful infection. We establish a broad functional interaction network of Qst, which includes enzymes of cofactor biosynthesis pathways (CoaC/ThiD) and a non-ribosomal peptide synthetase pathway (PA1217). Qst therefore represents an exquisite example of intricate reprogramming of the bacterium by a phage, which may be further exploited as tool to combat antibiotic resistant bacterial pathogens.&lt;/p&gt;
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