<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Normand, Anne-Cécile</style></author><author><style face="normal" font="default" size="100%">Sébastien Imbert</style></author><author><style face="normal" font="default" size="100%">Piarroux, Renaud</style></author><author><style face="normal" font="default" size="100%">Arnaud Fekkar</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Sophie Brun</style></author></secondary-authors><tertiary-authors><author><style face="normal" font="default" size="100%">Abdullah M S Al-Hatmi,</style></author></tertiary-authors><subsidiary-authors><author><style face="normal" font="default" size="100%">Erja Chryssanthou</style></author><author><style face="normal" font="default" size="100%">Sophie Cassaing</style></author><author><style face="normal" font="default" size="100%">Christine Schuttler</style></author><author><style face="normal" font="default" size="100%">Lilia Hasseine</style></author><author><style face="normal" font="default" size="100%">Caroline Mahinc</style></author><author><style face="normal" font="default" size="100%">Damien Costa</style></author><author><style face="normal" font="default" size="100%">Christine Bonnal</style></author><author><style face="normal" font="default" size="100%">Ranque, Stéphane</style></author><author><style face="normal" font="default" size="100%">Marc Sautour</style></author><author><style face="normal" font="default" size="100%">Elisa Rubio</style></author><author><style face="normal" font="default" size="100%">Delhaes, Laurence</style></author><author><style face="normal" font="default" size="100%">Arnaud Riat</style></author><author><style face="normal" font="default" size="100%">Boualem Sendid</style></author><author><style face="normal" font="default" size="100%">Lise Kristensen</style></author><author><style face="normal" font="default" size="100%">Marcel Brandenberger</style></author><author><style face="normal" font="default" size="100%">Juliette Guitard</style></author><author><style face="normal" font="default" size="100%">Ann Packeu</style></author></subsidiary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Clinical Origin and Species Distribution of  spp. Isolates Identified by Molecular Sequencing and Mass Spectrometry: A European Multicenter Hospital Prospective Study.</style></title><secondary-title><style face="normal" font="default" size="100%">J Fungi (Basel)</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2021 Mar 25</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">7</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any isolates encountered either in human pathology or in the environment.&lt;/p&gt;
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