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Résultats de la recherche - 20 results
Benchmarking bacterial taxonomic classification using nanopore metagenomics data of several mock communities
sequencing. While second-generation Illumina sequencing still dominates, third-generation nanopore sequencing promises improved classification through longer reads. However, extensive benchmarking studies on ...
Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.
of tiled amplicons. The resulting amplicons can be sequenced on all currently available high-throughput sequencing platforms. As an example, we have included a detailed protocol for performing nanopore ...
Metabarcoding and metagenomics techniques in monitoring airborne pollen
called “third generation sequencing” such as “PromethION”, “GridION” and “MinION” from “Oxford Nanopore Technology” and “PacBio CCS ” from “Pacific Biosciences”, generate much longer reads (LR) than those ...
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.
Stefan; Nancy Roosens; De Keersmaecker, Sigrid C J; Vanneste, Kevin Source: J Clin Microbiol (2024) Keywords: Illumina nanopore sequencing outbreak public health whole-genome sequencing. Abstract: ...
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.
Stefan; Nancy Roosens; De Keersmaecker, Sigrid C J; Vanneste, Kevin Source: J Clin Microbiol (2024) Keywords: Illumina nanopore sequencing outbreak public health whole-genome sequencing. Abstract: ...
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.
Stefan; Nancy Roosens; De Keersmaecker, Sigrid C J; Vanneste, Kevin Source: J Clin Microbiol (2024) Keywords: Illumina nanopore sequencing outbreak public health whole-genome sequencing. Abstract: ...
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.
with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, long-read Oxford Nanopore Technologies (ONT) sequencing has gained prominence and may ...
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
analysing the DNA of the sample, it can identify the presence of various pathogens to the strain level. Moreover, adaptive sampling is a new tool proposed during nanopore sequencing to target certain DNA ...
Hemorrhagic bowel syndrome in dairy cattle: Gross, histological, and microbiological characterization.
originate from small mucosal erosions through dissecting hemorrhage within the lamina muscularis mucosae. No invasive fungal growth was observed in any tissue. Bacteriological cultivation and nanopore ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...