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Typing of Clostridium perfringens by in vitro amplification of toxin genes54

contained primers amplifying simultaneously a part of alpha-toxin, beta-toxin, epsilon-toxin and enterotoxin genes. In order to distinguish between toxinotype A and E, the l-toxin gene fragment must be ...

Role of the YadA protein in prevention of opsonization of Yersinia enterocolitica by C3b molecules56

b than their YadA+ parent. Gene yadA, cloned as a minimal polymerase chain reaction fragment and introduced in trans, complemented the mutations. Production of YadA by recombinant Escherichia coli LK111 ...

Identification of the aadB gene coding for the aminoglycoside-2"-O-nucleotidyltransferase, ANT(2"), by means of the polymerase chain reaction36645

encoding the aminoglycoside-2”-O-nucleotidyltransferase, ANT (2”). Two primers, delineating a DNA fragment of 188 bp, and a specific probe within this fragment were constructed, based on the nucleotide ...

Genome mapping of Clostridium perfringens strains with I-CeuI shows many virulence genes to be plasmid-borne53

Polymorphism,Restriction Fragment Length Print Research Research Support SB- IM Sites strain toxin virulence Abstract: The intron-encoded endonuclease I-CeuI from Chlamydomonas eugametos was shown to cleave the circular ...

MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market

unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by ...

Chapter Twenty Seven- Generation of immune globulin single variable domains by display technologies

Epitopes Genetic Glycoprotein Heavy-Chain Antibody Fragments Immune Globulin Single Variable Domains Laboratories llama nanobody ON Phage Display prevention Rabies Rabies virus Research Selection strain ...

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