<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Arisseto Pavesi,A.</style></author><author><style face="normal" font="default" size="100%">M.C. de Figueiredo Toledo</style></author><author><style face="normal" font="default" size="100%">Govaert,Y.</style></author><author><style face="normal" font="default" size="100%">Joris Van Loco</style></author><author><style face="normal" font="default" size="100%">Stéphanie Fraselle</style></author><author><style face="normal" font="default" size="100%">J-M Degroodt</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A modified sample preparation for acrylamide determination in cocoa and coffee products</style></title><secondary-title><style face="normal" font="default" size="100%">Food analytical methods</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acrylamide</style></keyword><keyword><style  face="normal" font="default" size="100%">Analyses</style></keyword><keyword><style  face="normal" font="default" size="100%">AS</style></keyword><keyword><style  face="normal" font="default" size="100%">Clean up</style></keyword><keyword><style  face="normal" font="default" size="100%">Cocoa</style></keyword><keyword><style  face="normal" font="default" size="100%">Combination</style></keyword><keyword><style  face="normal" font="default" size="100%">Complex Matrices</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">i</style></keyword><keyword><style  face="normal" font="default" size="100%">identification</style></keyword><keyword><style  face="normal" font="default" size="100%">Ion</style></keyword><keyword><style  face="normal" font="default" size="100%">IS</style></keyword><keyword><style  face="normal" font="default" size="100%">Laboratories</style></keyword><keyword><style  face="normal" font="default" size="100%">LC-MS/MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Limit of Detection</style></keyword><keyword><style  face="normal" font="default" size="100%">Liquid chromatography-tandem mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass Spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">method</style></keyword><keyword><style  face="normal" font="default" size="100%">ON</style></keyword><keyword><style  face="normal" font="default" size="100%">performance</style></keyword><keyword><style  face="normal" font="default" size="100%">PRODUCTS</style></keyword><keyword><style  face="normal" font="default" size="100%">protein</style></keyword><keyword><style  face="normal" font="default" size="100%">purification</style></keyword><keyword><style  face="normal" font="default" size="100%">relative</style></keyword><keyword><style  face="normal" font="default" size="100%">Reproducibility</style></keyword><keyword><style  face="normal" font="default" size="100%">Sample</style></keyword><keyword><style  face="normal" font="default" size="100%">Solutions</style></keyword><keyword><style  face="normal" font="default" size="100%">Solvents</style></keyword><keyword><style  face="normal" font="default" size="100%">time</style></keyword><keyword><style  face="normal" font="default" size="100%">VALIDATION</style></keyword><keyword><style  face="normal" font="default" size="100%">variation</style></keyword><keyword><style  face="normal" font="default" size="100%">work</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">0/0/2008</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">55</style></number><volume><style face="normal" font="default" size="100%">1</style></volume><pages><style face="normal" font="default" size="100%">49 - 55</style></pages><isbn><style face="normal" font="default" size="100%">1936-9751</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A modified sample preparation for acrylamide determination in difficult matrices such as cocoa and coffee by a liquid chromatography-tandem mass spectrometry method is presented in this work. For the sample pretreatment, different solvents, purification steps, and solid-phase extraction cartridges were evaluated. The analyses were carried out on a µ-Bondapak C18 column using acrylamide-D3 as internal standard. For identification and confirmation of acrylamide, relative retention time and two diagnostic ions were monitored. Good performance of the method was achieved by introducing a protein precipitation step with Carrez I and II solutions and a liquid-liquid extraction with ethyl acetate in combination with solid-phase extraction during the sample clean-up. A limit of detection of 10 µg/kg, a limit of quantitation of 20 µg/kg, mean recoveries ranging from 93 to 99%, coefficients of variation of 3.4% for repeatability, and from 1.7 to 10.8% for within-laboratory reproducibility were obtained during a laboratory validation procedure.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom1><style face="normal" font="default" size="100%">2018</style></custom1><section><style face="normal" font="default" size="100%">49</style></section></record></records></xml>