<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sylvia Broeders</style></author><author><style face="normal" font="default" size="100%">Ingrid Huber</style></author><author><style face="normal" font="default" size="100%">L. Grohmann</style></author><author><style face="normal" font="default" size="100%">Berben,G.</style></author><author><style face="normal" font="default" size="100%">Isabel Taverniers</style></author><author><style face="normal" font="default" size="100%">Mazzara,M.</style></author><author><style face="normal" font="default" size="100%">Nancy Roosens</style></author><author><style face="normal" font="default" size="100%">Morisset,D.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Guidelines for validation of qualitative real-time PCR methods</style></title><secondary-title><style face="normal" font="default" size="100%">Trends in Food Science &amp; Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">an</style></keyword><keyword><style  face="normal" font="default" size="100%">approach</style></keyword><keyword><style  face="normal" font="default" size="100%">approaches</style></keyword><keyword><style  face="normal" font="default" size="100%">Area</style></keyword><keyword><style  face="normal" font="default" size="100%">Areas</style></keyword><keyword><style  face="normal" font="default" size="100%">AS</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">genetically</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetically modified</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetically modified organism</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetically modified organisms</style></keyword><keyword><style  face="normal" font="default" size="100%">GMO</style></keyword><keyword><style  face="normal" font="default" size="100%">GMO detection</style></keyword><keyword><style  face="normal" font="default" size="100%">GMO screening</style></keyword><keyword><style  face="normal" font="default" size="100%">Guidelines</style></keyword><keyword><style  face="normal" font="default" size="100%">implement</style></keyword><keyword><style  face="normal" font="default" size="100%">IS</style></keyword><keyword><style  face="normal" font="default" size="100%">method</style></keyword><keyword><style  face="normal" font="default" size="100%">methods</style></keyword><keyword><style  face="normal" font="default" size="100%">ON</style></keyword><keyword><style  face="normal" font="default" size="100%">PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">qPCR</style></keyword><keyword><style  face="normal" font="default" size="100%">qualitative</style></keyword><keyword><style  face="normal" font="default" size="100%">real time PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Real-time PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">Real-Time Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">SCREENING</style></keyword><keyword><style  face="normal" font="default" size="100%">Screening method</style></keyword><keyword><style  face="normal" font="default" size="100%">specific</style></keyword><keyword><style  face="normal" font="default" size="100%">study</style></keyword><keyword><style  face="normal" font="default" size="100%">technology</style></keyword><keyword><style  face="normal" font="default" size="100%">TESTING</style></keyword><keyword><style  face="normal" font="default" size="100%">VALIDATION</style></keyword><keyword><style  face="normal" font="default" size="100%">Validation study</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">0/0/2014</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">126</style></number><volume><style face="normal" font="default" size="100%">37</style></volume><pages><style face="normal" font="default" size="100%">115 - 126</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary.</style></abstract><custom1><style face="normal" font="default" size="100%">4790</style></custom1><section><style face="normal" font="default" size="100%">115</style></section></record></records></xml>