<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">M. Fischer</style></author><author><style face="normal" font="default" size="100%">Wernike,K.</style></author><author><style face="normal" font="default" size="100%">Freuling,C.M.</style></author><author><style face="normal" font="default" size="100%">Muller,T.</style></author><author><style face="normal" font="default" size="100%">Aylan,O.</style></author><author><style face="normal" font="default" size="100%">Bernard Brochier</style></author><author><style face="normal" font="default" size="100%">Cliquet,F.</style></author><author><style face="normal" font="default" size="100%">Vazquez-Moron,S.</style></author><author><style face="normal" font="default" size="100%">Hostnik,P.</style></author><author><style face="normal" font="default" size="100%">Huovilainen,A.</style></author><author><style face="normal" font="default" size="100%">Isaksson,M.</style></author><author><style face="normal" font="default" size="100%">Kooi,E.A.</style></author><author><style face="normal" font="default" size="100%">Mooney,J.</style></author><author><style face="normal" font="default" size="100%">Turcitu,M.</style></author><author><style face="normal" font="default" size="100%">Rasmussen,T.B.</style></author><author><style face="normal" font="default" size="100%">Revilla-Fernandez,S.</style></author><author><style face="normal" font="default" size="100%">Smreczak,M.</style></author><author><style face="normal" font="default" size="100%">Fooks,A.R.</style></author><author><style face="normal" font="default" size="100%">Marston,D.A.</style></author><author><style face="normal" font="default" size="100%">Beer,M.</style></author><author><style face="normal" font="default" size="100%">Hoffmann,B.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories33977</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS ONE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">2010</style></keyword><keyword><style  face="normal" font="default" size="100%">a</style></keyword><keyword><style  face="normal" font="default" size="100%">accuracy</style></keyword><keyword><style  face="normal" font="default" size="100%">ALL</style></keyword><keyword><style  face="normal" font="default" size="100%">an</style></keyword><keyword><style  face="normal" font="default" size="100%">application</style></keyword><keyword><style  face="normal" font="default" size="100%">article</style></keyword><keyword><style  face="normal" font="default" size="100%">AS</style></keyword><keyword><style  face="normal" font="default" size="100%">assessment</style></keyword><keyword><style  face="normal" font="default" size="100%">at</style></keyword><keyword><style  face="normal" font="default" size="100%">bat</style></keyword><keyword><style  face="normal" font="default" size="100%">Combination</style></keyword><keyword><style  face="normal" font="default" size="100%">Comparison</style></keyword><keyword><style  face="normal" font="default" size="100%">Concordance</style></keyword><keyword><style  face="normal" font="default" size="100%">conventional</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">detection method</style></keyword><keyword><style  face="normal" font="default" size="100%">diagnostic accuracy</style></keyword><keyword><style  face="normal" font="default" size="100%">Diagnostics</style></keyword><keyword><style  face="normal" font="default" size="100%">differences</style></keyword><keyword><style  face="normal" font="default" size="100%">disease</style></keyword><keyword><style  face="normal" font="default" size="100%">diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">dye</style></keyword><keyword><style  face="normal" font="default" size="100%">electronic</style></keyword><keyword><style  face="normal" font="default" size="100%">ET</style></keyword><keyword><style  face="normal" font="default" size="100%">European</style></keyword><keyword><style  face="normal" font="default" size="100%">Evolution</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">genomic</style></keyword><keyword><style  face="normal" font="default" size="100%">Germany</style></keyword><keyword><style  face="normal" font="default" size="100%">im</style></keyword><keyword><style  face="normal" font="default" size="100%">improve</style></keyword><keyword><style  face="normal" font="default" size="100%">Institute</style></keyword><keyword><style  face="normal" font="default" size="100%">International</style></keyword><keyword><style  face="normal" font="default" size="100%">IS</style></keyword><keyword><style  face="normal" font="default" size="100%">IT</style></keyword><keyword><style  face="normal" font="default" size="100%">journal</style></keyword><keyword><style  face="normal" font="default" size="100%">KNOWLEDGE</style></keyword><keyword><style  face="normal" font="default" size="100%">Laboratories</style></keyword><keyword><style  face="normal" font="default" size="100%">LEVEL</style></keyword><keyword><style  face="normal" font="default" size="100%">Lyssavirus</style></keyword><keyword><style  face="normal" font="default" size="100%">method</style></keyword><keyword><style  face="normal" font="default" size="100%">methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">ON</style></keyword><keyword><style  face="normal" font="default" size="100%">PARTICIPANTS</style></keyword><keyword><style  face="normal" font="default" size="100%">performance</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Rabies</style></keyword><keyword><style  face="normal" font="default" size="100%">Rabies virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Research</style></keyword><keyword><style  face="normal" font="default" size="100%">Research Support</style></keyword><keyword><style  face="normal" font="default" size="100%">result</style></keyword><keyword><style  face="normal" font="default" size="100%">Rna</style></keyword><keyword><style  face="normal" font="default" size="100%">Sample</style></keyword><keyword><style  face="normal" font="default" size="100%">Samples</style></keyword><keyword><style  face="normal" font="default" size="100%">SB - IM</style></keyword><keyword><style  face="normal" font="default" size="100%">SENSITIVITY</style></keyword><keyword><style  face="normal" font="default" size="100%">STANDARD</style></keyword><keyword><style  face="normal" font="default" size="100%">standards</style></keyword><keyword><style  face="normal" font="default" size="100%">System</style></keyword><keyword><style  face="normal" font="default" size="100%">Systems</style></keyword><keyword><style  face="normal" font="default" size="100%">Test</style></keyword><keyword><style  face="normal" font="default" size="100%">TESTING</style></keyword><keyword><style  face="normal" font="default" size="100%">use</style></keyword><keyword><style  face="normal" font="default" size="100%">varieties</style></keyword><keyword><style  face="normal" font="default" size="100%">variety</style></keyword><keyword><style  face="normal" font="default" size="100%">Version</style></keyword><keyword><style  face="normal" font="default" size="100%">virology</style></keyword><keyword><style  face="normal" font="default" size="100%">VIRUS</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">0/0/2013</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">e58372</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Rabies is a lethal and notifiable zoonotic disease for which diagnostics have to meet the highest standards. In recent years, an evolution was especially seen in molecular diagnostics with a wide variety of different detection methods published. Therefore, a first international ring trial specifically designed on the use of reverse transcription polymerase chain reaction (RT-PCR) for detection of lyssavirus genomic RNA was organized. The trial focussed on assessment and comparison of the performance of conventional and real-time assays. In total, 16 European laboratories participated. All participants were asked to investigate a panel of defined lyssavirus RNAs, consisting of Rabies virus (RABV) and European bat lyssavirus 1 and 2 (EBLV-1 and -2) RNA samples, with systems available in their laboratory. The ring trial allowed the important conclusion that conventional RT-PCR assays were really robust assays tested with a high concordance between different laboratories and assays. The real-time RT-PCR system by Wakeley et al. (2005) in combination with an intercalating dye, and the combined version by Hoffmann and co-workers (2010) showed good sensitivity for the detection of all RABV samples included in this test panel. Furthermore, all used EBLV-specific assays, real-time RT-PCRs as well as conventional RT-PCR systems, were shown to be suitable for a reliable detection of EBLVs. It has to be mentioned that differences were seen in the performance between both the individual RT-PCR systems and the laboratories. Laboratories which used more than one molecular assay for testing the sample panel always concluded a correct sample result. Due to the markedly high genetic diversity of lyssaviruses, the application of different assays in diagnostics is needed to achieve a maximum of diagnostic accuracy. To improve the knowledge about the diagnostic performance proficiency testing at an international level is recommended before using lyssavirus molecular diagnostics e.g. for confirmatory testing</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom1><style face="normal" font="default" size="100%">38192</style></custom1><section><style face="normal" font="default" size="100%">e58372</style></section></record></records></xml>