<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Torfs,H.</style></author><author><style face="normal" font="default" size="100%">J Poels</style></author><author><style face="normal" font="default" size="100%">Detheux,M.</style></author><author><style face="normal" font="default" size="100%">Dupriez,V.</style></author><author><style face="normal" font="default" size="100%">T. Van Loy</style></author><author><style face="normal" font="default" size="100%">Vercammen,L.</style></author><author><style face="normal" font="default" size="100%">Vassart,G.</style></author><author><style face="normal" font="default" size="100%">Parmentier,M.</style></author><author><style face="normal" font="default" size="100%">J. Vanden Broeck</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+ -levels in Drosophila S2 cells4412</style></title><secondary-title><style face="normal" font="default" size="100%">Invert.Neurosci.</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">0</style></keyword><keyword><style  face="normal" font="default" size="100%">a</style></keyword><keyword><style  face="normal" font="default" size="100%">Aequorin</style></keyword><keyword><style  face="normal" font="default" size="100%">agonists</style></keyword><keyword><style  face="normal" font="default" size="100%">an</style></keyword><keyword><style  face="normal" font="default" size="100%">analogs &amp; derivatives</style></keyword><keyword><style  face="normal" font="default" size="100%">analysi</style></keyword><keyword><style  face="normal" font="default" size="100%">analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">antagonists &amp; inhibitors</style></keyword><keyword><style  face="normal" font="default" size="100%">Apoproteins</style></keyword><keyword><style  face="normal" font="default" size="100%">article</style></keyword><keyword><style  face="normal" font="default" size="100%">AS</style></keyword><keyword><style  face="normal" font="default" size="100%">Belgium</style></keyword><keyword><style  face="normal" font="default" size="100%">Biology</style></keyword><keyword><style  face="normal" font="default" size="100%">Calcium</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Change</style></keyword><keyword><style  face="normal" font="default" size="100%">Changes</style></keyword><keyword><style  face="normal" font="default" size="100%">cytology</style></keyword><keyword><style  face="normal" font="default" size="100%">Drosophila</style></keyword><keyword><style  face="normal" font="default" size="100%">Drosophila melanogaster</style></keyword><keyword><style  face="normal" font="default" size="100%">effect</style></keyword><keyword><style  face="normal" font="default" size="100%">effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Functional</style></keyword><keyword><style  face="normal" font="default" size="100%">genetically</style></keyword><keyword><style  face="normal" font="default" size="100%">genetically engineered</style></keyword><keyword><style  face="normal" font="default" size="100%">i</style></keyword><keyword><style  face="normal" font="default" size="100%">im</style></keyword><keyword><style  face="normal" font="default" size="100%">Insect Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Institute</style></keyword><keyword><style  face="normal" font="default" size="100%">Intracellular Membranes</style></keyword><keyword><style  face="normal" font="default" size="100%">IS</style></keyword><keyword><style  face="normal" font="default" size="100%">journal</style></keyword><keyword><style  face="normal" font="default" size="100%">Laboratories</style></keyword><keyword><style  face="normal" font="default" size="100%">LEVEL</style></keyword><keyword><style  face="normal" font="default" size="100%">levels</style></keyword><keyword><style  face="normal" font="default" size="100%">Luminescent Measurements</style></keyword><keyword><style  face="normal" font="default" size="100%">means</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">method</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular biology</style></keyword><keyword><style  face="normal" font="default" size="100%">Monitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">ON</style></keyword><keyword><style  face="normal" font="default" size="100%">Order</style></keyword><keyword><style  face="normal" font="default" size="100%">Osmolar Concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">p</style></keyword><keyword><style  face="normal" font="default" size="100%">pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Print</style></keyword><keyword><style  face="normal" font="default" size="100%">protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptor</style></keyword><keyword><style  face="normal" font="default" size="100%">receptors</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptors,Invertebrate Peptide</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptors,Tachykinin</style></keyword><keyword><style  face="normal" font="default" size="100%">recombinant</style></keyword><keyword><style  face="normal" font="default" size="100%">Recombinant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Research</style></keyword><keyword><style  face="normal" font="default" size="100%">Research Support</style></keyword><keyword><style  face="normal" font="default" size="100%">response</style></keyword><keyword><style  face="normal" font="default" size="100%">Responses</style></keyword><keyword><style  face="normal" font="default" size="100%">SB - IM</style></keyword><keyword><style  face="normal" font="default" size="100%">study</style></keyword><keyword><style  face="normal" font="default" size="100%">Substance P</style></keyword><keyword><style  face="normal" font="default" size="100%">System</style></keyword><keyword><style  face="normal" font="default" size="100%">Tachykinins</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year><pub-dates><date><style  face="normal" font="default" size="100%">0/4/2002</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">124</style></number><volume><style face="normal" font="default" size="100%">4</style></volume><pages><style face="normal" font="default" size="100%">119 - 124</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The bioluminescent Ca(2+)-sensitive reporter protein, aequorin, was employed to develop an insect cell-based functional assay system for monitoring receptor-mediated changes of intracellular Ca(2)(+)-concentrations. Drosophila Schneider 2 (S2) cells were genetically engineered to stably express both apoaequorin and the insect tachykinin-related peptide receptor, STKR. Lom-TK III, an STKR agonist, was shown to elicit concentration-dependent bioluminescent responses in these S2-STKR-Aeq cells. The EC(50) value for the calcium effect detected by means of aequorin appeared to be nearly identical to the one that was measured by means of Fura-2, a fluorescent Ca(2)(+)-indicator. In addition, this aequorin-based method was also utilised to study receptor antagonists. Experimental analysis of the effects exerted by spantide I, II and III, three potent substance P antagonists, on Lom-TK III-stimulated S2-STKR-Aeq cells showed that these compounds antagonise STKR-mediated responses in a concentration-dependent manner. The rank order of inhibitory potencies was spantide III &gt; spantide II &gt; spantide I</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom1><style face="normal" font="default" size="100%">4412</style></custom1><section><style face="normal" font="default" size="100%">119</style></section></record></records></xml>