<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Muriel Vincent</style></author><author><style face="normal" font="default" size="100%">Marta Romano</style></author><author><style face="normal" font="default" size="100%">Corazza,F.</style></author><author><style face="normal" font="default" size="100%">K Huygen</style></author><author><style face="normal" font="default" size="100%">Michel,O.</style></author><author><style face="normal" font="default" size="100%">Olivier J Denis</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a dot-blot assay for the detection of mould-specific IgE in the Belgian population</style></title><secondary-title><style face="normal" font="default" size="100%">Mycopathologia</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">a</style></keyword><keyword><style  face="normal" font="default" size="100%">Belgian</style></keyword><keyword><style  face="normal" font="default" size="100%">Belgian population</style></keyword><keyword><style  face="normal" font="default" size="100%">detection</style></keyword><keyword><style  face="normal" font="default" size="100%">Development</style></keyword><keyword><style  face="normal" font="default" size="100%">IgE</style></keyword><keyword><style  face="normal" font="default" size="100%">POPULATION</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">04/2017</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">329</style></number><volume><style face="normal" font="default" size="100%">182</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Background&lt;/p&gt;

&lt;p&gt;Data on mould sensitization in the general population are scarce and mostly on&amp;nbsp;Aspergillus fumigatus, Alternaria alternata&amp;nbsp;and&amp;nbsp;Cladosporium herbarum.&lt;/p&gt;

&lt;p&gt;Objectives&lt;/p&gt;

&lt;p&gt;To validate a dot-blot assay for the detection of specific IgE and evaluate the prevalence of mould sensitization in a healthy population.&lt;/p&gt;

&lt;p&gt;Methods&lt;/p&gt;

&lt;p&gt;The dot-blot assay was validated against the CAP test. Sensitization rate to ten common indoor and outdoor mould species in 344 serum samples was calculated. For each serum with more than one reactivity, the &amp;ldquo;major sensitization&amp;rdquo; defined as the strongest response against a single mould species was calculated.&lt;/p&gt;

&lt;p&gt;Results&lt;/p&gt;

&lt;p&gt;Intra- and inter-assay variations were both below 20%, and the positivity threshold of the test was of 0.418&amp;nbsp;kU/L for&amp;nbsp;A. fumigatus. Correlation with CAP results was strong. The overall prevalence of sensitization was 32.8%, and the commonest sensitizations were against&amp;nbsp;A. alternaria, A. flavus&amp;nbsp;and&amp;nbsp;A. niger&amp;nbsp;(around 15%). The most frequent &amp;ldquo;major reactivities&amp;rdquo; were against&amp;nbsp;A. niger&amp;nbsp;and&amp;nbsp;A. alternata&amp;nbsp;(20&amp;ndash;30%). In 25.1% of the samples, &amp;ldquo;major reactivities&amp;rdquo; were directed against a group of moulds commonly found indoor (Penicillium&amp;nbsp;spp., Aspergillus versicolor, Cladosporium sphaerospermum&amp;nbsp;and&amp;nbsp;Cladosporium cladosporioides).&lt;/p&gt;

&lt;p&gt;Conclusions&lt;/p&gt;

&lt;p&gt;The dot-blot assay was validated for the detection of mould-specific IgE. In the general population, sensitization to indoor species was common and accounted for 25% of overall mould sensitizations.&lt;/p&gt;
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