<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Boxus, Mathieu</style></author><author><style face="normal" font="default" size="100%">Marylène Tignon</style></author><author><style face="normal" font="default" size="100%">S. Roels</style></author><author><style face="normal" font="default" size="100%">Toussaint, Jean-François</style></author><author><style face="normal" font="default" size="100%">Walravens, Karl</style></author><author><style face="normal" font="default" size="100%">Benoit, Marie-Ange</style></author><author><style face="normal" font="default" size="100%">Coppe, Philippe</style></author><author><style face="normal" font="default" size="100%">Letesson, Jean-Jacques</style></author><author><style face="normal" font="default" size="100%">Letellier, Carine</style></author><author><style face="normal" font="default" size="100%">Pierre Kerkhofs</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">DNA immunization with plasmids encoding fusion and nucleocapsid proteins of bovine respiratory syncytial virus induces a strong cell-mediated immunity and protects calves against challenge.</style></title><secondary-title><style face="normal" font="default" size="100%">J Virol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J. Virol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Antibodies, Viral</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle</style></keyword><keyword><style  face="normal" font="default" size="100%">Cattle Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Cercopithecus aethiops</style></keyword><keyword><style  face="normal" font="default" size="100%">COS Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunity, Cellular</style></keyword><keyword><style  face="normal" font="default" size="100%">Interferon-gamma</style></keyword><keyword><style  face="normal" font="default" size="100%">mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice, Inbred BALB C</style></keyword><keyword><style  face="normal" font="default" size="100%">Nucleocapsid Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Respiratory Syncytial Virus Infections</style></keyword><keyword><style  face="normal" font="default" size="100%">Respiratory Syncytial Virus, Bovine</style></keyword><keyword><style  face="normal" font="default" size="100%">Vaccines, DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Vero Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Fusion Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Vaccines</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007 Jul</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">81</style></volume><pages><style face="normal" font="default" size="100%">6879-89</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Respiratory syncytial viruses (RSV) are one of the most important respiratory pathogens of humans and cattle, and there is currently no safe and effective vaccine prophylaxis. In this study, we designed two codon-optimized plasmids encoding the bovine RSV fusion (F) and nucleocapsid (N) proteins and assessed their immunogenicity in young calves. Two administrations of both plasmids elicited low antibody levels but primed a strong cell-mediated immunity characterized by lymphoproliferative response and gamma interferon production in vitro and in vivo. Interestingly, this strong cellular response drastically reduced viral replication, clinical signs, and pulmonary lesions after a highly virulent challenge. Moreover, calves that were further vaccinated with a killed-virus vaccine developed high levels of neutralizing antibody and were fully protected following challenge. These results indicate that DNA vaccination could be a promising alternative to the classical vaccines against RSV in cattle and could therefore open perspectives for vaccinating young infants.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">13</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17459933?dopt=Abstract</style></custom1></record></records></xml>