<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Weydert, Caroline</style></author><author><style face="normal" font="default" size="100%">Bart van Heertum</style></author><author><style face="normal" font="default" size="100%">Lieve Dirix</style></author><author><style face="normal" font="default" size="100%">Stéphanie De Houwer</style></author><author><style face="normal" font="default" size="100%">Flore De Wit</style></author><author><style face="normal" font="default" size="100%">Jan Mast</style></author><author><style face="normal" font="default" size="100%">Steven J Husson</style></author><author><style face="normal" font="default" size="100%">Katrien Busschots</style></author><author><style face="normal" font="default" size="100%">Renate König</style></author><author><style face="normal" font="default" size="100%">Gijsbers, Rik</style></author><author><style face="normal" font="default" size="100%">De Rijck, Jan</style></author><author><style face="normal" font="default" size="100%">Debyser, Zeger</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Y-box-binding protein 1 supports the early and late steps of HIV replication.</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS One</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2018</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The human immunodeficiency virus (HIV) depends on cellular proteins, so-called cofactors, to complete its replication cycle. In search for new therapeutic targets we identified the DNA and RNA binding protein Y-box-binding Protein 1 (YB-1) as a cofactor supporting early and late steps of HIV replication. YB-1 depletion resulted in a 10-fold decrease in HIV-1 replication in different cell lines. Dissection of the replication defects revealed that knockdown of YB-1 is associated with a 2- to 5-fold decrease in virion production due to interference with the viral RNA metabolism. Using single-round virus infection experiments we demonstrated that early HIV-1 replication also depends on the cellular YB-1 levels. More precisely, using quantitative PCR and an in vivo nuclear import assay with fluorescently labeled viral particles, we showed that YB-1 knockdown leads to a block between reverse transcription and nuclear import of HIV-1. Interaction studies revealed that YB-1 associates with integrase, although a direct interaction with HIV integrase could not be unambiguously proven. In conclusion, our results indicate that YB-1 affects multiple stages of HIV replication. Future research on the interaction between YB-1 and the virus will reveal whether this protein qualifies as a new antiviral target.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">7</style></issue></record></records></xml>