<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">An Van den Bossche</style></author><author><style face="normal" font="default" size="100%">Hugo Varet</style></author><author><style face="normal" font="default" size="100%">Amandine Sury</style></author><author><style face="normal" font="default" size="100%">Odile Sismeiro</style></author><author><style face="normal" font="default" size="100%">Rachel Legendre</style></author><author><style face="normal" font="default" size="100%">Jean-Yves Coppee</style></author><author><style face="normal" font="default" size="100%">Vanessa Mathys</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Transcriptional profiling of a laboratory and clinical Mycobacterium tuberculosis strain suggests respiratory poisoning upon exposure to delamanid</style></title><secondary-title><style face="normal" font="default" size="100%">Tuberculosis</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Delamanid</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycobacterium tuberculosis</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Transciptomics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-07-2019</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">117</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Tuberculosis (TB) is the most deadly infectious disease worldwide. To reduce TB incidence and counter the&lt;/p&gt;

&lt;p&gt;spread of multidrug resistant TB, the discovery and characterization of new drugs is essential. In this study, the&lt;/p&gt;

&lt;p&gt;transcriptional response of two Mycobacterium tuberculosis strains to a pressure of the recently approved delamanid&lt;/p&gt;

&lt;p&gt;is investigated. Total RNA sequencing revealed that the response to this bicyclic nitroimidazole shows&lt;/p&gt;

&lt;p&gt;many similarities with pretomanid, an anti-tuberculous drug from the same class. Although delamanid is found&lt;/p&gt;

&lt;p&gt;to inhibit cell wall synthesis, the expression of genes involved in this process were only mildly affected. In&lt;/p&gt;

&lt;p&gt;contrast, a clear parallel was found with components that affect aerobic respiration. This demonstrates that,&lt;/p&gt;

&lt;p&gt;besides the inhibition of cell wall synthesis, respiratory poisoning plays a fundamental role in the bactericidal&lt;/p&gt;

&lt;p&gt;effect of delamanid. Remarkably, the most highly induced genes comprise poorly characterized genes for which&lt;/p&gt;

&lt;p&gt;functional characterization might hint to the target molecule(s) of delamanid and its exact mode(s) of action.&lt;/p&gt;
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