<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ti Thu Ha Nguyen</style></author><author><style face="normal" font="default" size="100%">M. Ledent</style></author><author><style face="normal" font="default" size="100%">Véronique Beauvois</style></author><author><style face="normal" font="default" size="100%">Anthonissen, Roel</style></author><author><style face="normal" font="default" size="100%">Luc Verschaeve</style></author><author><style face="normal" font="default" size="100%">Jean-Francois Collard</style></author><author><style face="normal" font="default" size="100%">Maurice Hinsenkamp</style></author><author><style face="normal" font="default" size="100%">Veronique Feipel</style></author><author><style face="normal" font="default" size="100%">Birgit Mertens</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro 50 Hz magnetic field long-term exposure: Cytogenetic tests on human lymphoblastoid TK6 cells and validation of the test environment</style></title><secondary-title><style face="normal" font="default" size="100%">MethodsX</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Background magnetic field</style></keyword><keyword><style  face="normal" font="default" size="100%">Comet Assay</style></keyword><keyword><style  face="normal" font="default" size="100%">Extremely low-frequency magnetic fields</style></keyword><keyword><style  face="normal" font="default" size="100%">Micronucleus test</style></keyword><keyword><style  face="normal" font="default" size="100%">Shielding system</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-01-2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">7</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined&amp;nbsp;in vitro&amp;nbsp;comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well.&lt;/p&gt;

&lt;p&gt;&amp;nbsp;&lt;/p&gt;

&lt;ul&gt;
	&lt;li&gt;Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results.&lt;/li&gt;
	&lt;li&gt;Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs.&lt;/li&gt;
	&lt;li&gt;A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels.&lt;/li&gt;
&lt;/ul&gt;
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