<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">De Smet, Jeroen</style></author><author><style face="normal" font="default" size="100%">Jeroen Wagemans</style></author><author><style face="normal" font="default" size="100%">Maarten Boon</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">Voet, Marleen</style></author><author><style face="normal" font="default" size="100%">Noben, Jean-Paul</style></author><author><style face="normal" font="default" size="100%">Julia Andreeva</style></author><author><style face="normal" font="default" size="100%">Dmitry Ghilarov</style></author><author><style face="normal" font="default" size="100%">Konstantin Severinov</style></author><author><style face="normal" font="default" size="100%">Lavigne, Rob</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The bacteriophage LUZ24 &quot;Igy&quot; peptide inhibits the Pseudomonas DNA gyrase.</style></title><secondary-title><style face="normal" font="default" size="100%">Cell Rep</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacteriophage</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Gyrase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2021 Aug 24</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">36</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The bacterial DNA gyrase complex (GyrA/GyrB) plays a crucial role during DNA replication and serves as a target for multiple antibiotics, including the fluoroquinolones. Despite it being a valuable antibiotics target, resistance emergence by pathogens including Pseudomonas aeruginosa are proving problematic. Here, we describe Igy, a peptide inhibitor of gyrase, encoded by Pseudomonas bacteriophage LUZ24 and other members of the Bruynoghevirus genus. Igy (5.6&amp;nbsp;kDa) inhibits in&amp;nbsp;vitro gyrase activity and interacts with the P.&amp;nbsp;aeruginosa GyrB subunit, possibly by DNA mimicry, as indicated by a de novo model of the peptide and mutagenesis. In&amp;nbsp;vivo, overproduction of Igy blocks DNA replication and leads to cell death also in fluoroquinolone-resistant bacterial isolates. These data highlight the potential of discovering phage-inspired leads for antibiotics development, supported by co-evolution, as Igy may serve as a scaffold for small molecule mimicry to target the DNA gyrase complex, without cross-resistance to existing molecules.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue></record></records></xml>