<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pirnay, Jean-Paul</style></author><author><style face="normal" font="default" size="100%">Sarah Djebara</style></author><author><style face="normal" font="default" size="100%">Griet Steurs</style></author><author><style face="normal" font="default" size="100%">Johann Griselain</style></author><author><style face="normal" font="default" size="100%">Cochez, Christel</style></author><author><style face="normal" font="default" size="100%">Steven De Soir</style></author><author><style face="normal" font="default" size="100%">Tea Glonti</style></author><author><style face="normal" font="default" size="100%">An Spiessens</style></author><author><style face="normal" font="default" size="100%">Emily Vanden Berghe</style></author><author><style face="normal" font="default" size="100%">Sabrina Green</style></author><author><style face="normal" font="default" size="100%">Jeroen Wagemans</style></author><author><style face="normal" font="default" size="100%">Lood, Cédric</style></author><author><style face="normal" font="default" size="100%">Eddie Schrevens</style></author><author><style face="normal" font="default" size="100%">Chanishvili, Nina</style></author><author><style face="normal" font="default" size="100%">Mzia Kutateladze</style></author><author><style face="normal" font="default" size="100%">Mathieu de Jode</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">Jean-Pierre Draye</style></author><author><style face="normal" font="default" size="100%">Verbeken, Gilbert</style></author><author><style face="normal" font="default" size="100%">De Vos, Daniel</style></author><author><style face="normal" font="default" size="100%">Rose, Thomas</style></author><author><style face="normal" font="default" size="100%">Jolien Onsea</style></author><author><style face="normal" font="default" size="100%">Brieuc Van Nieuwenhuyse</style></author><author><style face="normal" font="default" size="100%">Patrick Soentjens</style></author><author><style face="normal" font="default" size="100%">Lavigne, Rob</style></author><author><style face="normal" font="default" size="100%">Merabishvili, Maya</style></author></authors><translated-authors><author><style face="normal" font="default" size="100%">Bacteriophage Therapy Providers</style></author><author><style face="normal" font="default" size="100%">Bacteriophage Donors</style></author></translated-authors></contributors><titles><title><style face="normal" font="default" size="100%">Personalized bacteriophage therapy outcomes for 100 consecutive cases: a multicentre, multinational, retrospective observational study.</style></title><secondary-title><style face="normal" font="default" size="100%">Nat Microbiol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AMR</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteriophage therapy</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2024 Jun</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In contrast to the many reports of successful real-world cases of personalized bacteriophage therapy (BT), randomized controlled trials of non-personalized bacteriophage products have not produced the expected results. Here we present the outcomes of a retrospective observational analysis of the first 100 consecutive cases of personalized BT of difficult-to-treat infections facilitated by a Belgian consortium in 35 hospitals, 29 cities and 12 countries during the period from 1 January 2008 to 30 April 2022. We assessed how often personalized BT produced a positive clinical outcome (general efficacy) and performed a regression analysis to identify functional relationships. The most common indications were lower respiratory tract, skin and soft tissue, and bone infections, and involved combinations of 26 bacteriophages and 6 defined bacteriophage cocktails, individually selected and sometimes pre-adapted to target the causative bacterial pathogens. Clinical improvement and eradication of the targeted bacteria were reported for 77.2% and 61.3% of infections, respectively. In our dataset of 100 cases, eradication was 70% less probable when no concomitant antibiotics were used (odds ratio = 0.3; 95% confidence interval = 0.127-0.749). In vivo selection of bacteriophage resistance and in vitro bacteriophage-antibiotic synergy were documented in 43.8% (7/16 patients) and 90% (9/10) of evaluated patients, respectively. We observed a combination of antibiotic re-sensitization and reduced virulence in bacteriophage-resistant bacterial isolates that emerged during BT. Bacteriophage immune neutralization was observed in 38.5% (5/13) of screened patients. Fifteen adverse events were reported, including seven non-serious adverse drug reactions suspected to be linked to BT. While our analysis is limited by the uncontrolled nature of these data, it indicates that BT can be effective in combination with antibiotics and can inform the design of future controlled clinical trials. BT100 study, ClinicalTrials.gov registration: NCT05498363 .&lt;/p&gt;
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