Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression. [1]
Peer reviewed scientific article
SCIENSANO
Auteurs
De Boever, S [2]; Croubels, S [3]; Demeyere, K [4]; Bénédicte Lambrecht [5]; De Backer, P [6]; Meyer, E [7]Trefwoorden
Samenvatting:
A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-positive cells was confirmed by sorting the subpopulations and subsequent light microscopic evaluation. In these differentiated cell populations, intracellular expression analysis of the proinflammatory cy…