High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei. [1]
Peer reviewed scientific article
Engels
SCIENSANO
Auteurs
G Girault [2]; P Wattiau [3]; M Saqib [4]; B Martin [5]; F Vorimore [6]; H Singha [7]; M Engelsma [8]; H J Roest [9]; S Spicic [10]; R Grunow [11]; N Vicari [12]; Sigrid C.J. De Keersmaecker [13]; Nancy Roosens [14]; M Fabbi [15]; B N Tripathi [16]; Zientara, S [17]; N Madani [18]; K Laroucau [19]Trefwoorden
Samenvatting:
Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analys…