Search results - 100 results
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control.
reaction (real-time RT- PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house ...
Clinical prediction rules combining signs, symptoms and epidemiological context to distinguish influenza from influenza-like illnesses in primary care: a cross sectional study36562
signs, general characteristics and contextual information. The samples were analysed by a DirectigenFlu-A&B and RT- PCR tests. 4584 records were useful for further analysis.Starting from the most ...
Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance34020
participating laboratories; 10 laboratories returned quantitative data. All assays except one were developed in-house using conventional or real-time reverse transcriptase PCR (RT- PCR) methodologies. There was ...
Oral fluid for the serological and molecular diagnosis of measles.
reverse transcriptase polymerase chain reaction (RT- PCR) using nasopharyngeal secretions. METHODS: Three samples each from 73 measles-positive and 44 measles-negative subjects (serum, oral fluid, and ...
Effect of PstS sub-units or PknD deficiency on the survival of Mycobacterium tuberculosis.
provides energy through ATP hydrolysis. In the Mycobacterium tuberculosis genome, four different gene clusters encode three PstS, and two of each of the other sub-units. We used RT- PCR to show that these ...
A duplex real-time RT-PCR for the detection of bluetongue virus in bovine semen.
https://www.sciensano.be/en/biblio/a-duplex-real-time-rt-pcr-detection-bluetongue-virus-bovine-semen
(BTV). The aim of this study was to validate a real-time RT- PCR for the direct testing of semen for artificial insemination (AI). The amplification of the BTV target was combined with an internal control ...
Limited genetic diversity and gene expression differences between egg- and non-egg-related Salmonella Enteritidis strains36855
mRNA expression levels of fimA and agfA under conditions of growth simulating the conditions during egg formation were determined by real-time RT- PCR. Although differences in fimA and agfA expression ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...
Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.
real-time PCR (RT- PCR) tests for the fine quantification of mutations in a background of wild-type sequences. As a proof-of-concept model, we developed mutation-specific RT- PCR tests to quantify antibody ...
Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.
Chain Reaction Specific Pathogen-Free Organisms Abstract: A real-time reverse transcription PCR (RRT- PCR) targeting a highly conserved HA2 H7 region was developed for the detection of all H7 subtype ...