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A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
comparison with the manual protocol. These parameters give essential information about the performance of the robot and are of great importance when the automated assay is used in an accreditation system ...
Development of specific enzyme-linked immunosorbent assays to evaluate the duck immune response after experimental infection with H5N1 and H7N1 low pathogenic avian influenza viruses.
immunosorbent assays (ELISA s) for duck IgY, Ig light chain, IgY (heavy chain), IgA, and IgM were developed and used to evaluate the systemic and mucosal response induced in ducks after low pathogenic avian ...
Evaluation of different serologic markers for the early detection of avian influenza infection in chickens.
important as early warning systems, and they can also be valuable tools as companion tests of vaccines (differentiating infected from vaccinated animals), but they might also be informative about the ...
Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox.
in partial nitritation/anammox systems to remove ammonium from wastewater. In this process, large granular microbial aggregates enhance the performance, but little is known about granulation so far. In ...
A pyrazolotriazolopyrimidinamine inhibitor of bovine viral diarrhea virus replication that targets the viral RNA-dependent RNA polymerase.
interaction between F224 side chain and the ring system of LZ37 and (iii) a hydrogen bond between the amino function of LZ37 and the O backbone atom of A392. It is concluded that LZ37 interacts with the same ...
The major portal of entry of koi herpesvirus in Cyprinus carpio is the skin.
further demonstrate the role of the skin as the KHV portal of entry, we constructed an original system, nicknamed “U-tube,” to perform percutaneous infection restricted to the posterior part of the fish. ...
NUSAP method for evaluating the data quality in a quantitative microbial risk assessment model for Salmonella in the pork production chain.
assessment pedigree (NUSAP) system was implemented to evaluate the quality of input parameters in a quantitative microbial risk assessment (QMRA) model for Salmonella spp. in minced pork meat. The input ...
Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI.
response and the lowest antibody increase after challenge were observed in the group of ducks whose immune system was primed with the fowlpox vectored vaccine and boosted with the inactivated vaccine, ...
Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.
that the system has been validated beforehand with a significant number of strains originating from that particular area. Health Topics: Animal health Category: A1 FOS Classification: 4.03 Veterinary ...
Possible routes of introduction of bluetongue virus serotype 8 into the epicentre of the 2006 epidemic in north-western Europe.
transfers of domestic or wild ruminants, non-susceptible mammal species and ruminant germ plasms into the AFI during the high-risk period (HRP), registered by the Trade Control and Expert System (TRACES) of ...