Search results - 48 results

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

method targeting the  B. subtilis  group, including  B. subtilis,  B. licheniformis,  B. amyloliquefaciens  and  B. velezensis. The method’s performance was successfully assessed as specific and sensitive, ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

low concentrations. Comparable results were obtained using short-read sequencing, without being able to link the identified species with the detected AMR genes. Our approach was also successful on ...

Organizing the health interview survey at the local level: design of a pilot study.

younger adults, and women more than men. CONCLUSION: The LHIS can be successfully implemented in Flemish municipalities. The method, however, does not guarantee that the composition of the final sample ...

Movements of free-range pigs in rural communities in Zambia: an explorative study towards future ring interventions for the control of Taenia solium

transmission and, consequently, the success of ring interventions against T. solium. The aim of the present study was to explore roaming patterns of pigs in T. solium endemic communities in Zambia as a first ...

COVID-19 contact tracing in Belgium: main indicators and performance, January- September 2021.

of reported HRC was 2.7. In total, 666.869 HRC were reported of which 91% were successfully contacted and 89% of these were tested at least once following the interview. The estimated average secondary ...

Development, validation and application of multi-class methods for the analysis of food additives by liquid chromatography coupled to tandem mass spectrometry

quantification, accuracy, repeatability, and intra-laboratory reproducibility. Finally, the methods were successfully applied to eighty-four actual samples. All additives were found below authorised levels. ...

Development of a data-intensive centralized system for surveillance and outbreak investigation of bacterial pathogens using whole-genome sequencing

sequencing (WGS), the sequencing of (nearly) complete genomes, has had an enormous impact on almost all fields of biology. However, its successful integration into routine public health practice has lagged ...

No molecular detection of tick-borne pathogens in the blood of patients with erythema migrans in Belgium

methods, and repetition of the DNA extraction procedure and qPCR test was not successful. The qPCR test results for the other tick-borne pathogens were negative. Conclusions In general, only a few patients ...

In Vivo Kinetics and Biotransformation of Aflatoxin B1 in Dairy Cows Based on the Establishment of a Reliable UHPLC-MS/MS MethodDataSheet1.docx

successfully validated by the determination of linearity (R2  ≥ 0.990), sensitivity (lower limit of quantification, 0.1–0.2 ng ml−1), recovery (79.5–111.2%), and precision relative standard deviation (RSD) ...

WGS- versus ORF5-Based Typing of PRRSV: A Belgian Case Study.

results show that (nearly) complete PRRSV genomes can be obtained directly from serum samples with a high success rate. Analysis of the coding regions confirmed the exceptionally high genetic diversity, ...

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