PCR identification et sérotypage

Test description

• Description: Identification of Listeria monocytogenes and determination of serogroup based on classical in-house PCR. This test is performed on purified strains from which DNA is extracted, in case of difficulties in classical serotyping by agglutination.
• Result: identification of Listeria monocytogenes and determination of serogroup.

Purpose

Confirmation of the identification and serogrouping of isolated Listeria monocytogenes strains.

Criteria for performing this test in the context of reference activities

Confirmation of Listeria identification and serogrouping of samples isolated from patients showing symptoms of listeriosis (analysis performed in the framework of NRC Listeria surveillance).

Instruction for samples

•     Type: Isolated strains of Listeria on a transport medium or other solid medium. Exceptionally, a DNA extract of the sample may also be sent. 
•     Minimum quantity: Not applicable

Transport instructions

The strain is sent at room temperature and must be packed in a double protective container for safety reasons. This container must be hermetically sealed.

Unacceptable applications

Not applicable

Turn around time (and frequency of analysis)

• Turnaround time: 10 working days.
• Analysis days: Any weekday (excluding Public holidays, extended weekends and the period between Christmas and New Year).

Reporting of test results

The analysis report will be sent in the manner desired by the applicant.