Résultats de la recherche - 145 results
Some guidelines for determining foot-and-mouth disease vaccine strain matching by serology.
of foot-and-mouth disease (FMD) virus can be assessed in vivo or by serological r-value determination. Sera from animals involved in vaccine potency and cross-protection trials performed using the ...
The polymeric stability of the Escherichia coli F4 (K88) fimbriae enhances its mucosal immunogenicity following oral immunization.
subunit FaeG that is also the fimbrial adhesin. In the present study, the mutagenesis experiments identified FaeG amino acids 97 (N to K) and 201 (I to V) as determinants for F4 polymeric stability. The ...
Brucella suis identification and biovar typing by real-time PCR.
described ones in real-time PCR assays applicable to low-biosafety conditions. Allelic profiles unique for each B. suis biovar were defined and the most relevant signatures were determined on a collection of ...
Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants.
reaction RNA, Ribosomal, 18S Sequence Analysis, DNA Abstract: The diversity of free-living protozoa in five meat-cutting plants was determined. Light microscopy after enrichment culturing was combined with ...
Indirect foot-and-mouth disease vaccine potency testing based on a serological alternative.
laboratories and assays used, the majority of antibody pass-levels, determined using ROC analysis, corresponded to at least 75.0% probability of protection. The indirect potency assessment procedure proposed is ...
Occurrence and genetic diversity of Bacillus anthracis strains isolated in an active wool-cleaning factory.
processed material (South Caucasian and Middle Eastern), all B. anthracis isolates belonged to the same phylogenetic cluster, as determined by variable-number tandem repeat (VNTR) typing at eight loci. Within ...
Application of a Commercial Real-Time RT-PCR Assay for Surveillance of Classical Swine Fever: Evaluation by Testing Sequential Tissue and Blood Samples
WIL ’). Based on the viral distribution determined in tissue by the real-time RT- PCR assay (rRT- PCR), the tonsils were found the most suitable tissue for CSFV detection. In the tonsils, the viral ...
Foot-and-mouth disease vaccine potency testing in cattle using homologous and heterologous challenge strains: precision of the "Protection against Podal Generalisation" test.
relatedness of the vaccine strain and circulating field isolate. The “Gold Standard” FMD vaccine potency test is the in vivo test performed in the target species. The objective of the study was to determine the ...
Expression of reelin, its receptors and its intracellular signaling protein, Disabled1 in the canary brain: relationships with the song control system.
incorporation of new neurons. Reelin, a large glycoprotein defective in reeler mice, is assumed to determine the final location of migrating neurons in the developing brain. In mammals, reelin is also expressed ...
Uncertainty of measurement for competitive and indirect ELISAs.
disease (FMD) ELISA s. Assay repeatability was determined by random effects analysis of variance, and the normality of the residuals was checked. The standard errors of the individual predicted values were ...