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Résultats de la recherche - 24 results
Molecular characterization of Belgian pseudorabies virus isolates from domestic swine and wild boar.
the Grand Duchy of Luxembourg were genetically characterized by restriction fragment length polymorphism (RFLP) analysis and phylogenetic analysis. While all wild boar isolates were characterized as ...
False-positive results in metagenomic virus discovery: a strong case for follow-up diagnosis.
targeting the detected sequence fragments confirmed the presence of these sequences in the original sample as well as in a sample of an additional animal, but a contamination with an identical genetic ...
Towards a typing strategy for Arcobacter species isolated from humans and animals and assessment of the in vitro genomic stability.
Amplified Fragment Length Polymorphism Analysis Animals Arcobacter Bacterial Typing Techniques Cattle Cluster Analysis DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Feces Food Microbiology Genomic ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
sequence depth. We confirmed this finding experimentally using both fragmented and non-fragmented viral RNA s as well as primers differing in random oligomer length (6 or 12 nucleotides) and in the sequence ...
Occurrence and diversity of free-living protozoa on butterhead lettuce.
Identification of FLP was performed by light microscopy and sequencing of denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments. Bodo saltans, Spumella (-like) spp. and Cercozoa were the ...
No H- and L-type cases in Belgium in cattle diagnosed with bovine spongiform encephalopathy (1999-2008) aging seven years and older.
types behave as different strains of BSE and were named H-type and L-type according to the high and low molecular mass of the unglycosylated fragment of their proteinase K resistant prion protein ...
Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants.
sequencing of PCR-amplified, denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments, which was used as a fast screening method. The general results of the survey showed that ...
Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.
strains. Therefore these two methods are complementary and could be used in parallel to confirm the diagnosis of a possible new introduction of BTV. An RT-qPCR amplifying a fragment of the beta-actin mRNA ...
Kinetic and biologic properties of recombinant ChIFN-gamma expressed via CELO-virus vector.
cytomegalovirus (CMV) promoter. The ChIFN-gamma expression cassette was inserted in the right end of the CELO genome (D fragment), which was able to carry the largest insertion of foreign DNA without affecting the ...
Characterisation of the discrepancy between PCR and virus isolation in relation to classical swine fever virus detection.
PCR was used. Furthermore, the primer-dependent amplification, most likely caused by the presence of viral fragments, demonstrates the necessity of confirming a single positive PCR result, certainly in ...