Zoekresultaten - 28 results
The impact of ecosystem nitrogen enrichment on pollen allergy: a cross-sectional paired comparison study
nitrogen-enriched (fertilised) and non-enriched common semi-natural grasslands in Belgium. Pollen from paired grasslands (n=50, enriched [n=25] vs non-enriched [n=25]) based on their common geography, were sampled ...
Impact of environmental nitrogen enrichment on birch pollen allergy
a gradient of environmental nitrogen based on nitrogen deposition map data (Eutrophication caused by atmospheric nitrogen deposition in Europe, European Environment Agency, EEA). Nitrogen deposition values ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
based on the sequencing of all genetic material of the sample without isolation has become available some years ago, e.g. shotgun metagenomics. It allows to get a screenshot of every microbiological ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...
Capacity building in European health information systems: the InfAct peer assessment methodology.
based on 12 semi-structured interviews. RESULTS: The main advantage of the assessments is its informal atmosphere, high degree of objectiveness and its networking opportunities. Disadvantages are its ...
Clinical and analytical evaluation of the Alinity m HR HPV assay within the VALGENT-3 framework
population-based cervical screening program enriched with 300 cytologically abnormal samples. Overall hrHPV test concordance was assessed by kappa values; the concordance for HPV-16 and HPV-18 was assessed for ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
species, present in a microbial community, which has up until now not been demonstrated for this application. The current work shows the feasibility of strain-level metagenomics of enriched food matrix ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
outbreak depends on the rapid and accurate identification of the responsible food source. Conventional methods based on isolation of the pathogen from the food matrix and target-specific real-time polymerase ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
Microbiology and Biotechnology, Volume 102, Issue 7, Number 3285 (2018) Keywords: Detection; Pathogenic E. coli; Real-time PCR; STEC; SYBR® Green; Validation Abstract: A detection and discrimination system for ...
Performance of a Cartridge-Based Assay for Detection of Clinically Significant Human Papillomavirus (HPV) Infection: Lessons from VALGENT (Validation of HPV Genotyping Tests).
performance. Through VALGENT, we assessed the performance of the cartridge-based Xpert HPV assay (Xpert HPV), which detects 14 high-risk (HR) types and resolves HPV16 and HPV18 /45. Samples from women attending ...