Zoekresultaten - 35 results
Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance34020
participating laboratories; 10 laboratories returned quantitative data. All assays except one were developed in-house using conventional or real-time reverse transcriptase PCR (RT- PCR) methodologies. There was ...
Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells.
real-time PCR. Multiplication of avian viruses was cold-sensitive, whatever cell type. By contrast, temperature sensitivity of swine viruses was found to depend on the virus and the host cell: for an H1N1 ...
Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR36884
Quantitative real-time PCR real time PCR Real-time PCR Research Research Support result results Sample Samples SB- IM Sheep Strategies Strategy study Swine The Netherlands Toxoplasma Transmission Type Abstract: ...
Use of pJANUS-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment.
constructed. The dynamic range, efficiency and limit of detection for the soybean event GTS-40-3-2 real-time quantitative polymerase chain reaction (Q- PCR) system described by Terry et al. (J AOAC Int ...
Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 136839
A quantitative two-step multiplex real-time reverse transcriptase (RT-) PCR assay for the simultaneous detection of genogroup I (GI) and genogroup II (GII) noroviruses (NoVs) is described below. A murine norovirus ...
Humoral, cell-mediated and mucosal immunity induced by oculo-nasal vaccination of one-day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines.
real-time reverse transcription-polymerase chain reaction (QRRT- PCR). The interference of maternally derived antibody with vaccination was also considered in conventional layer chickens. In SPF chickens, ...
Laboratory efforts to eliminate contamination problems in the real-time RT-PCR detection of noroviruses36840
laboratory efforts to prevent the presence of positive NTC s (no template controls) during the optimization of a quantitative real-time reverse transcriptase PCR assay for detection of Noroviruses (NoVs) are ...
Quantification of Campylobacter spp. in chicken carcass rinse by real-time PCR36795
Reaction SAFETY SB- IM SENSITIVITY Sensitivity and Specificity Statistical study technology Abstract: AIMS: In this study, a real-time quantitative polymerase chain reaction (PCR) method was examined for its ...
Bluetongue in Belgium, 2006.
Abstract: Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCR s (RT-qPCRs) that ...
Survival and stress induced expression of groEL and rpoD of Campylobacter jejuni from different growth phases36763
Oxygen PCR pharmacology polymerase chain reaction Print Quantitative real-time PCR real time PCR Real-time PCR Research Research Support resistance Reverse Transcriptase Polymerase Chain Reaction Rna ...