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Zoekresultaten - 27 results
Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.
strains. Therefore these two methods are complementary and could be used in parallel to confirm the diagnosis of a possible new introduction of BTV. An RT-qPCR amplifying a fragment of the beta-actin mRNA ...
Kinetic and biologic properties of recombinant ChIFN-gamma expressed via CELO-virus vector.
cytomegalovirus (CMV) promoter. The ChIFN-gamma expression cassette was inserted in the right end of the CELO genome (D fragment), which was able to carry the largest insertion of foreign DNA without affecting the ...
Characterisation of the discrepancy between PCR and virus isolation in relation to classical swine fever virus detection.
PCR was used. Furthermore, the primer-dependent amplification, most likely caused by the presence of viral fragments, demonstrates the necessity of confirming a single positive PCR result, certainly in ...
Chicken recombinant antibodies specific for very virulent infectious bursal disease virus.
Abstract: A phage-displayed single chain variable fragment (scFv) antibody library was constructed from the immune spleen cells of chickens immunized with very virulent infectious bursal disease virus ...
Comparison of molecular techniques for the typing of Mycoplasma hyopneumoniae isolates.
Swine, Mycoplasmal Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Reproducibility of Results Sequence Analysis, DNA Swine Abstract: In this study, we compared the ...
Host- and tissue-specific pathogenic traits of Staphylococcus aureus.
colonized or infected humans. A total of 77 veterinary isolates were genetically characterized by high-throughput amplified fragment length polymorphism (AFLP). Bacterial genotypes were introduced in a large ...
Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species.
the restriction endonuclease AluI (AG – CT) was found to be most discriminative and generated from 3 to 13 fragments depending on the Mycoplasma species. Although 73 Mycoplasma species could be ...
Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting.
to selectively amplify a fragment of the Acinetobacter 16S rRNA gene from environmental samples by PCR. 16S rRNA gene products were only obtained in PCR s with DNA from members of the genus ...
Phylogenetic analysis of fowl adenoviruses.
2, p.164-70 (2004) Keywords: Adenoviridae Adenoviridae Infections Animals Capsid Proteins Europe Phylogeny polymerase chain reaction Polymorphism, Restriction Fragment Length Poultry Poultry Diseases ...
Evaluation of virulence of Mycoplasma hyopneumoniae field isolates.
Based on the results of the different parameters, isolates were divided into three “virulence” groups: low, moderately and highly virulent strains. Typically, a 5000 bp RAPD fragment was associated with ...