Zoekresultaten - 58 results
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Multivalent binding of herpesvirus to living cells is tightly regulated during infection.
introduce a new method to force-probe multivalent attachment directly on living cells, and we show, for the first time, direct evidence of a new mechanism by which a herpesvirus surface glycoprotein acts as ...
Time-and-motion tool for the assessment of working time in tuberculosis laboratories: a multicentre study.
Processing specimens in batches could help save time in highly automated assays (e.g., line-probe) (TTPS 00:14:00 vs. 00:09:45 for batches comprising 7 and 31 specimens, respectively). CONCLUSIONS: The T&M ...
Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.
Publication Type: Peer reviewed scientific article Authors: Agianniotaki, Eirini I; Chaintoutis, Serafeim C; Andy Haegeman; Tasioudi, Konstantia E; Ilse De Leeuw; Katsoulos, Panagiotis-Dimitrios; Sachpatzidis, Achilleas; Kris De Clercq; Alexandropoulos, T ...
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
biotinylated probes with the capture of those probes with streptavidin-coated paramagnetic beads, T. gondii DNA can selectively be “fished out” from a large volume of meat lysate. Still, several studies showed ...
Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.
(2017) Keywords: Air Pollution, Indoor DNA Probes environment Fungi Limit of Detection Nucleic Acid Hybridization Abstract: Considered as a public health problem, indoor fungal contamination is generally ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
multiplex oligonucleotide ligation- PCR procedure was used, with read-out on a Luminex MAGPIX (®) platform. We designed 46 xTAG(®)-compatible probes targeting β-lactamase alleles and allele variants, and one ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
ten avian gene primer and probe sets that can be used to identify suitable reference genes in many experimental contexts. The panel was tested with TaqMan and SYBR Green systems in two experimental ...
Optimized MOL-PCR for Characterization of Microbial Pathogens
Oligonucleotide Probes Phycoerythrin polymerase chain reaction Polymorphism, Single Nucleotide Salmonella typhimurium Streptavidin Temperature Abstract: Characterization of microbial pathogens is necessary for ...
Non-surgical periodontal therapy with systemic antibiotics in patients with untreated aggressive periodontitis: a systematic review and meta-analysis.
for additional information. The following outcome measures were analysed: mean probing pocket depth reduction, mean clinical attachment level gain and mean bleeding on probing change. Extracted data ...