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Zoekresultaten - 46 results
Use of continuous results to compare ELISAs for the detection of antibodies to non-structural proteins of foot-and-mouth disease virus.
international workshop in Brescia, Italy in 2004 on the basis of dichotomous test results. However, as results from all of these assays were also available on a continuous scale, validation was extended by ...
Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus.
Publication Type: Peer reviewed scientific article Authors: Le Dimna, M; Vrancken, R; F. Koenen; Bougeard, S; Mesplède, A; Hutet, E; Kuntz-Simon, G; Le Potier, M F Source: J Virol Methods, Volume 147, Issue 1, p.136-42 (2008) Keywords: Animals Classical S ...
An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen.
uninfected seronegative bulls, negative semen spiked with virus, as well as serial dilutions of reference virus. The samples were tested using a previously validated real-time PCR assay for the detection of ...
Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen.
detection of the presence of bovine herpesvirus type 1 (BoHV-1) in extended bovine semen. The assay detects a region encoding a highly conserved glycoprotein B gene. The real-time PCR assay was validated for ...
A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.
tissue samples was used to validate the endogenous control. The application of an endogenous internal control proves an excellent strategy both for avoiding false negative diagnostic results and for ...
Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.
labour-intensive. Molecular diagnosis is also complex because of the high genetic diversity between and within the 24 serotypes of BTV. In the present study, two laboratories joined forces to develop and validate two ...
Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus.
Cattle Databases, Factual Enzyme-Linked Immunosorbent Assay Foot-and-Mouth Disease Virus Reproducibility of Results Sheep Swine Vaccination Viral Proteins Abstract: To validate the use of serology in ...
Genetic variability of encephalomyocarditis virus (EMCV) isolates.
the virus under environmental changes (passages), along with its wide distribution among vertebrate species (including higher primates), shows the validity of considering EMCV as a potential pathogen ...
Ochratoxin A and citrinin loads in stored wheat grains: impact of grain dust and possible prediction using ergosterol measurement.
content as a general marker of fungal biomass. Present results suggest that this predictive tool would only be valid for predicting the contamination level of CIT and OTA at reasonable moisture content ...
Quality assurance/quality control of foot and mouth disease solid phase competition enzyme-linked immunosorbent assay--Part II. Quality control: comparison of two charting methods to monitor assay performance.
Reproducibility of Results Sensitivity and Specificity Vaccines, Marker Viral Vaccines Abstract: Diagnostic laboratories are increasingly required to meet stringent quality standards, and validated assays are ...