Search results - 35 results
Metagenomics-based tracing of genetically modified microorganism contaminations in commercial fermentation products
phylogenomic approaches. In most cases, analysis of 16 GMM-contaminated food enzyme products supported finding the same GM producer strains in different products, while often multiple GMM contaminations per ...
Pilot market surveillance of GMM contaminations in alpha-amylase food enzyme products: A detection strategy strengthened by a newly developed qPCR method targeting a GM Bacillus licheniformis producing alpha-amylase
Publication Type: Peer reviewed scientific article Authors: Fraiture, Marie-Alice; Gobbo, Andrea; Guillitte, Chloé; Marchesi, Ugo; Verginelli, Daniela; De Greve, Joke; D'aes, Jolien; Vanneste, Kevin; N. Papazova; Roosens, Nancy H.C. Source: Food Chem ...
Plants with stacked genetically modified events: to assess or not to assess?
Number 73, p.70-3 (2014) Keywords: Genetically modified organism Safety assessment stacked GM event Abstract: The principles for the safety assessment of genetically modified (GM) organisms (GMO s) are ...
Characterization of Genetically Modified Microorganisms Using Short- and Long-Read Whole-Genome Sequencing Reveals Contaminations of Related Origin in Multiple Commercial Food Enzyme Products.
10, Issue 11 (2021) Abstract: Despite their presence being unauthorized on the European market, contaminations with genetically modified (GM) microorganisms have repeatedly been reported in diverse ...
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
fermentation products on the market is prohibited by European regulations. GMM s are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
Abstract: In Belgium, an official control plan was established in 2016 to detect the potential presence of an unauthorized genetically modified (GM) Bacillus subtilis RASFF2014.1249 strain in commercialized ...
Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus
(GM) Bacillus subtilis isolated as unexpected contamination in a feed additive, and therefore considered unauthorized (RASFF 2014.1249), as a case study. In GM organisms, AMR genes are used as selection markers. ...
Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes
feasibility using several GM events possessing these CryAb/c genes. Afterwards, its sensitivity has been investigated through low concentrations of targets (as low as 20 HGE). In addition, to illustrate its ...
Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.
Specificity Sequence Analysis, DNA Abstract: BACKGROUND: Recently, the presence of an unauthorized genetically modified (GM) Bacillus subtilis bacterium overproducing vitamin B2 in a feed additive was notified ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/en/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification), simultaneous ...