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Replication Characteristics of African Swine Fever Virus (ASFV) Genotype I E70 and ASFV Genotype II Belgium 2018/1 in Perivenous Macrophages Using Established Vein Explant Model.
being present close to the vein (within a radius of 0-348 µm). Upon inoculation with G1- E70 and G2- B18, we observed an increase in the quantity of cells testing positive for viral antigens over time. ...
Genomic comparison between and and analysis of peptide-based biomarkers for serodiagnosis.
proteins. From the 28 proteins, 157 B-cell epitope-based peptides were identified that discriminated between and species. Although confirmation by testing is still required, these candidate synthetic peptides ...
Lessons Learned from Active Clinical and Laboratory Surveillance during the Sheep Pox Virus Outbreak in Spain, 2022-2023.
swabs proved to be the sample of choice for early detection in the absence of scabs and could be tested in pools of five without extensive loss in sensitivity; serology by ELISA was not useful in outbreak ...
Allele Frequencies of Genetic Variants Associated with Varroa Drone Brood Resistance (DBR) in Apis mellifera Subspecies across the European Continent.
non-reproduction in drone brood were discovered in a single colony from the Amsterdam Water Dune population in the Netherlands. Recently, a new study tested the applicability of these eight genetic variants for the ...
PRRSV-Vaccinated, Seronegative Sows and Maternally Derived Antibodies (II): Impact on PRRSV-1 Vaccine Effectiveness and Challenge Outcomes in Piglets.
Syndrome virus (PRRSV) is widely practiced in both sows and piglets. However, it has been shown that multivaccinated sows sometimes lack a detectable antibody response, testing seronegative in ELISA ...
RNA sequencing of avian paramyxovirus (Paramyxoviridae, Avulavirinae) isolates from wild mallards in Belgium, 2021: complete genomes and coinfections.
strains and one avian Paraavulavirus-4 (APMV-4) strain were determined confirming hemagglutination inhibition testing of the virus isolates. In addition, the applied sequencing strategy identified an avian ...
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of ...
Report on initial findings of on-site diagnostic tests and IT solutions for human clinical, veterinary and environmental samples for early warning of emerging resistant pathogens
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Manal AbuOun; Indre Navickaite; Thomas I. Wilkes; Marco Di Domenico; Giuliano Garofolo; Bram Bloemen; Mathieu Gand; Nancy Roosens; Kevin Vanneste; Sigrid C.J. De Kee ...
Methodological choices in brucellosis burden of disease assessments: A systematic review
Most studies retrieved brucellosis epidemiological data from administrative registries. Incidence data were often estimated on the basis of laboratory-confirmed tests. Not all studies included mortality ...
Tick-Borne Encephalitis Virus Prevalence in Sheep, Wild Boar and Ticks in Belgium.
antibodies by ELISA and plaque reduction neutralization test (PRNT), respectively. The specificity of positive samples was assessed by PRNT s for TBEV and the Louping Ill, West Nile, and Usutu viruses. TBEV ...