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Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

health authorities. However, many clinical laboratories struggle to differentiate non- EIEC, EIEC, and Shigella in their current workflows, leading to inaccuracies in surveillance and rising numbers of ...

Towards the use of ion mobility mass spectrometry derived collision cross section as a screening approach for unambiguous identification of targeted pesticides in food

different screening workflows based on TWCCS, mass accuracy and retention times. Results The results proved that TWCCS values are very consistent, as the measured values do not differ more than 1% from the ...

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

this work, we have compared three existing SNP-based subtyping workflows using a benchmark dataset of 32 Salmonella enterica subsp. enterica serovar Typhimurium and serovar 1,4,[5],12:i:- isolates ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

applicability, the entire workflow has been tested on a sample mimicking food/feed matrices analysed in GMO routine analysis. Conclusion Given the successful assessment of its performance, the present ...

How can we better detect unauthorized GMO in the food and feed chain

6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria.

finding its way to current diagnostic workflows. However, further validations and automation are required before routine implementation can be envisioned. Health Topics:  Food consumption and food safety ...

Inter-laboratory study of human in vitro toxicogenomics-based tests as alternative methods for evaluating chemical carcinogenicity: a bioinformatics perspective.

Transcriptome Workflow Abstract: The assessment of the carcinogenic potential of chemicals with alternative, human-based in vitro systems has become a major goal of toxicogenomics. The central read-out of these ...

Chemometrics and the identification of counterfeit medicines-A review.

of actual case studies dealing with verifying the authenticity of selected medicines supported by different chemometric approaches. In particular, a general data processing workflow is discussed with ...

Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)

food/feed matrices, this strategy represents a key tool, easily implementable by the enforcement laboratories. With the aim to even more simplify the workflow and increase the throughput of this strategy, the ...

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