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A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates
genes which enable to distinguish field and vaccine strains. Based on this information, we developed and validated (n = 199) a Luminex-based assay targeting seven specific single-nucleotide polymorphisms. ...
Safety assessment of titanium dioxide (E171) as a food additive.
With respect to genotoxicity, the Panel concluded that TiO particles have the potential to induce DNA strand breaks and chromosomal damage, but not gene mutations. No clear correlation was observed ...
Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks
C.J. De Keersmaecker Source: Frontiers in Microbiology, Volume 12 (2021) Keywords: DNA extraction kits enterotoxin gene profiling outbreak investigation relatedness determination Staphylococcus aureus, ...
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
microbial fermentation products shotgun metagenomics Abstract: The presence of a genetically modified microorganism (GMM) or its DNA, often harboring antimicrobial resis- tance (AMR) genes, in microbial ...
Colistin resistance genes mcr-1 to mcr-5, including a case of triple occurrence (mcr-1,-3 and-5), in Escherichia coli isolates from faeces of healthy pigs, cattle and poultry in Belgium, 2012–2016
Publication Type: Peer reviewed scientific article Authors: Michaël Timmermans; P Wattiau; Denis, Olivier; Cécile Boland Source: International Journal of Antimicrobial Agents, Volume 57, Issue 6 (2021) Health Topics: Antibiorésistance Antimicrobial resis ...
First detection of a plasmid-encoded New-Delhi metallo-beta-lactamase-1 (NDM-1) producing Acinetobacter baumannii using whole genome sequencing, isolated in a clinical setting in Benin.
combatting increasing antibiotic resistance and development of new drugs. Since it was originally reported in Klebsiella pneumoniae in 2009, the quick spread of the bla gene encoding a New-Delhi ...
Safety assessment of titanium dioxide (E171) as a food additive
effects. With respect to genotoxicity, the Panel concluded that TiO2 particles have the potential to induce DNA strand breaks and chromosomal damage, but not gene mutations. No clear correlation was observed ...
Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants
Keersmaecker Source: Genes, Volume 12, Issue 4 (2021) Keywords: SARS-CoV-2; COVID-19; variants; detection; RT-qPCR; in silico specificity evaluation; bioinformatics tool; WGS data; mismatches; primers and probes ...
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
performs complete characterization of Shiga toxin-producing Escherichia coli (STEC) isolates including antimicrobial resistance prediction, virulence gene detection, serotype prediction, plasmid replicon ...
Identification of flavouring substances of genotoxic concern present in e-cigarette refills
induced gene mutations and chromosome damage in vitro, whereas equivocal results were obtained for the fourth compound. Thus, although there is a legislative framework which excludes the use of CMR ...