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Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
based on the sequencing of all genetic material of the sample without isolation has become available some years ago, e.g. shotgun metagenomics. It allows to get a screenshot of every microbiological ...
Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard
URL: https://doi.org/10.5281/zenodo.7429361 Keywords: Antimicrobial resistance Bacterial community Long read metagenomics Metagenome Health Topics: Health and disease monitoring Food consumption and ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
have had a breakthrough with the launch of nanopore sequencing, which through the production of long sequencing reads, portability and real-time data generation, represents a disruptive innovation for ...
Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies
Technologies, and we compared this to Illumina short-read-based metagenomics. After 12 h of MinION sequencing, strain-level characterization could be achieved, linking the food containing a pathogen to the ...