Search results - 8 results
Accuracy of immunological tests on serum and urine for diagnosis of Taenia solium neurocysticercosis: A systematic review
perform large-scale evaluations whilst holding into account both optimized test performance and ease of use. Accessibility to validated tests and feasibility of implementation should also be considered. ...
Introduction of WGS in food microbiology: advantages and challenges
implementation. As with the implementation of any method the validations of the WGS methodology requires a significant effort. Furthermore, the interpretation of the results should be done with care. Therefore, in ...
Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks
during SFP outbreak investigation. Although confirming the production of enterotoxins remains important for SFP investigation, we delivered a proof-of-concept that WGS is a valid alternative and/or ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
in the food vehicle without its prior isolation and link it back to human cases. We tested and validated a shotgun metagenomics approach by spiking food pathogens in specific food matrices using the ...
Pork as a source of transmission of Toxoplasma gondii to humans: a parasite burden study in pig tissues after infection with different strains of Toxoplasma gondii as a function of time and different parasite stages.
during the acute and chronic phases was studied. The parasite burden in different tissues was measured with a ISO 17025 validated Magnetic Capture-quantitative PCR. A high burden of infection was found in ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
Microbiology and Biotechnology, Volume 102, Issue 7, Number 3285 (2018) Keywords: Detection; Pathogenic E. coli; Real-time PCR; STEC; SYBR® Green; Validation Abstract: A detection and discrimination system for ...
A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods
well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology ...
RNA sequencing-based transcriptional overview of xerotolerance in Cronobacter sakazakii SP291.
PCR) was used to validate the RNA-seq data, we found that the primary desiccation response was gradually downregulated during the tested 4 hours of desiccation, while the secondary response remained ...