Search results - 9 results
Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing
curtail the virus spread. Additionally, emerging SARS-CoV-2 variants have increased the need for genomic surveillance to detect particular strains because of their potentially increased transmissibility, ...
Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.
SARS-CoV-2 for the selection of the two PCR assays targeting two different regions in the genome, and to monitor the possible impact of virus evolution on the specificity of the primers and probes of the PCR ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
and chikungunya (CHIKV) viruses simultaneously. Methods Two methods targeting different genome segments were selected from the literature for each virus. These were adapted for high genome coverage and ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, were selected from ...
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
18 published RT-qPCR methods using more than 3000 publically available genomes in the NCBI Virus Variation Resource, and provide a systematic overview of method performance based on in silico ...
Molecular and clinical investigation of Zika virus outbreak in New Caledonia
Molecular neurological non-invasive ON outbreak outbreak investigation pathogen public public health Public-health region result results Rna Sample Samples SENSITIVITY serum Spread strain time urine VIRUS WHO ...
The use of nucleic acid amplification techniques to increase the viral safety of blood.
Transfusion DNA, Viral Humans Nucleic Acid Amplification Techniques Virus Diseases Abstract: Despite recent significant improvements in the viral safety of blood and blood products there remains a small risk of ...
Proposal for Human Respiratory Syncytial Virus Nomenclature below the Species Level.
C Williams; Ian G. Barr; Ursula J Buchholz Source: Emerg Infect Dis, Volume 27, Issue 6 (2021) Keywords: Child Genetic Variation Genotype Humans Molecular Epidemiology Phylogeny Respiratory Syncytial Virus ...
Whole-genome-based phylogenomic analysis of the Belgian 2016-2017 influenza A(H3N2) outbreak season allows improved surveillance.
Winand; Saelens, Xavier; Nancy Roosens; Kevin Vanneste Source: Microb Genom, Volume 7, Issue 9 (2021) Keywords: Belgium Hemagglutinin Glycoproteins, Influenza Virus Humans Influenza A Virus, H3N2 Subtype ...