Zoekresultaten - 10 results
Occurrence of microcystin congeners in surface waters and food in Belgium as a source of exposure to humans
the producers of these toxins by amplification and sequencing of the mcyE gene. Yet, data on the MC s prevalence is not sufficient to accurately assess human exposure and risk. Additional consumption ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...
Development, evaluation and optimization of next generation sequencing data analysis tools in support of a fast response for public health and food chain safety
Publication Type: Dissertation Authors: Assia Saltykova Source: Ghent University, Ghent, Belgium, p.308 (2022) Keywords: bioinformatics tools Database GMO Metagenomics NGS outbreak investigation unauthorized GMO WGS-based subtyping Health Topics: Health ...
Development of a data-intensive centralized system for surveillance and outbreak investigation of bacterial pathogens using whole-genome sequencing
a limiting factor, as full runs on Illumina sequencers typically take several days to complete. Therefore, a real-time data analysis protocol for Illumina sequencing was implemented, enabling data analysis ...
Development of a genoserotyping system for the identification of Salmonella serotypes
detection of molecular markers or on the analysis of sequencing data, are described in the scientific literature. Based on a critical review of some of these techniques, the Multiplex Oligonucleotide ...
Fusarium, clinical relevance and mass spectrometry methods for identification, using strains of the Belgian fungal culture collection BCCM/IHEM
isolated from patients, were re-identified by multilocus DNA marker sequencing and phylogeny. The remaining 289 validated strains, comprising 40 different species, were of great value: (i) Analysis of the ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
analysis time. However, qPCR has some limitations especially concerning the discrimination of genetically close species and multiplexing. The first issue was addressed through the use of post-qPCR high ...
Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)
https://www.sciensano.be/nl/biblio/strategies-detect-unauthorized-gmo-food-and-feed-chain-phd-thesis
pCAMBIA), were selected to develop a strategy allowing to detect and characterize a broad range of GMO. This strategy, fully integrated in the GMO routine analysis, consists to characterize sequences ...
Strategies to detect unauthorized genetically modified organisms in food and feed chain
routine analysis, consists to characterize sequences surrounding detected key transgenic elements using a DNA walking approach. By this way, the acquisition of sequences from the junction between the ...
Exploring the added value of Whole Genome Sequencing in routine and pandemic viral surveillance
Publication Type: Dissertation Authors: Laura Van Poelvoorde Source: (2023) Abstract: Whole genome sequencing (WGS) has opened up a lot of new possibilities for virus surveillance, however, many ...